Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9284-IL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

NP_703190.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17RC Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-17 RC protein
Human IL-17 RC Isoform 1
(Leu21-His465)
Accession # NP_703190.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

76 kDa

SDS-PAGE

95-113 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-17RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # 7955-IL) binds with an ED50 = 0.1-0.6 ng/mL.

Scientific Data Images for Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein, CF

Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein Bioactivity

Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein Bioactivity

When Recombinant Human IL-17 RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # 7955-IL) binds with an ED50 = 0.1-0.6 ng/mL.

Formulation, Preparation and Storage

9284-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RC

IL-17 receptor C (IL-17RC; also known as IL-17RL and IL-17Rhom) is an 85-110 kDa member of the IL-17 receptor family. IL-17 plays a key role in the development of autoimmunity and inflammation (1, 2). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on neutrophils, endothelial cells, chondrocytes and osteoblasts, breast and prostatic epithelium, fibroblasts, renal tubular epithelium, and skeletal muscle cells (3-5). Mature human IL-17 RC consists of a 518 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region (6). It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (4, 7). This recombinant protein corresponds to IL-17 RC isoform 1, the canonical form. Within the ECD, IL-17 RC isoform 1 shares 69% aa sequence identity with mouse IL-17 RC. IL-17 RC is the cognate receptor for IL-17F, and it binds IL-17A with similar affinity (8). IL-17 RC associates wiith IL-17 RA to form a receptor complex for both IL-17A and IL-17F (8-11).

References

  1. Song, X. and Y. Qian (2013) Cytokine 62:175.
  2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
  3. Taylor, P.R. et al. (2014) Nat. Immunol. 15:143.
  4. You, Z. et al. (2007) Neoplasia 9:464.
  5. Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
  6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  7. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
  8. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  9. Toy, D. et al. (2006) J. Immunol. 177:36.
  10. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
  11. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.

Long Name

Interleukin 17 Receptor C, Transcript Variant 3

Alternate Names

IL-17 RC, IL-17 RL, IL-17RL, IL17RC

Entrez Gene IDs

84818 (Human); 171095 (Mouse)

Gene Symbol

IL17RC

UniProt

NP_703190.1

Additional IL-17RC Products

Product Documents for Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein, CF

For research use only

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