Recombinant Human IL-18 BPa Fc Chimera Protein, CF Best Seller
R&D Systems, part of Bio-Techne | Catalog # 119-BP
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IL-18 BPa protein
| Human IL-18 Bpa (Thr29-Gly192) Accession # AAD17190 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Thr29
Predicted Molecular Mass
44.2 kDa (monomer)
SDS-PAGE
60-80 kDa, reducing conditions
Activity
Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells.
The ED50 for this effect is 0.02‑0.12 µg/mL in the presence of 40 ng/mL of recombinant human IL-18.
The ED50 for this effect is 0.02‑0.12 µg/mL in the presence of 40 ng/mL of recombinant human IL-18.
Reviewed Applications
Read 1 review rated 4 using 119-BP in the following applications:
Formulation, Preparation and Storage
119-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-18 BPa
Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL-18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.
References
- Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
- Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
- Calderara, S. et al. (2001) Virology 279:22.
Long Name
Interleukin 18 Binding Protein Isoform a
Alternate Names
IL18 BPa, IL18BP
Gene Symbol
IL18BP
UniProt
Additional IL-18 BPa Products
Product Documents for Recombinant Human IL-18 BPa Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human IL-18 BPa Fc Chimera Protein, CF
For research use only
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