Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF
R&D Systems, part of Bio-Techne | Catalog # 816-LR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IL-18 R alpha/IL-1 R5 protein
| Human IL-18 R alpha (Glu20 - Arg329) Accession # Q13478 |
IEGRDID | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu20
Predicted Molecular Mass
63 kDa (monomer)
SDS-PAGE
95-105 kDa, reducing conditions
Activity
Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera (Catalog # 118-AP).
Approximately 5-15 µg/mL of rhIL-18 Ra/Fc will inhibit 50% of the biological response due to 40 ng/mL of rhIL-18 in the presence of 30 µg/mL of rhIL-18 R beta (AcPL)/Fc Chimera.
Approximately 5-15 µg/mL of rhIL-18 Ra/Fc will inhibit 50% of the biological response due to 40 ng/mL of rhIL-18 in the presence of 30 µg/mL of rhIL-18 R beta (AcPL)/Fc Chimera.
Formulation, Preparation and Storage
816-LR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 1 mg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-18 R alpha/IL-1 R5
Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.
References
- Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
- Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name
Interleukin 18 Receptor alpha
Alternate Names
CD218a, IL-1 R5, IL-1 Rrp, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1
Gene Symbol
IL18R1
UniProt
Additional IL-18 R alpha/IL-1 R5 Products
Product Documents for Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF
Product Specific Notices for Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF
For research use only
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