Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI11116
Biotinylated
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
HEK293
Accession #
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Biotinylated via Avi-tag
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human IL-1Rrp2/IL-1R6 protein
| Human IL-1 R6 (Asp20-Tyr337) Accession # Q9HB29.2 |
DIEGRMD | Human IgG1 Fc (Pro100-Lys330) |
Avi-tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Protein identity confirmed by mass spectrometry.
Predicted Molecular Mass
65 kDa (monomer)
SDS-PAGE
78-87 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB/CF) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag (Catalog # AVI11116) binds with an ED50 of 1.50-15.0 μg/mL.
When Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB/CF) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag (Catalog # AVI11116) binds with an ED50 of 1.50-15.0 μg/mL.
Scientific Data Images for Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF
Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag Protein Binding Activity.
When Recombinant Human IL-36 beta/IL-1F8 (6834-ILB/CF) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag Protein (Catalog # AVI11116) binds with an ED50 of 1.50-15.0 μg/mL.
Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Avi-tag Protein (Catalog # AVI11116) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-87 kDa and 156-174 kDa, respectively.Formulation, Preparation and Storage
AVI11116
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-1Rrp2/IL-1R6
The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1Rrp2 (IL-1 RL2/IL-1R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1Rrp2, respectively. IL-1Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1Rrp2 has been shown to mediate the activation of the transcription factor NF kappaB by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon), with IL-1RAcP as a cofactor (3, 4). Response to IL-1F9 is specifically antagonized by IL-1 F5 (also known as IL-1 delta), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1Rrp2, IL-1 F5, and IL-1F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1Rrp2 mediated signaling pathway may take part in local inflammatory responses (3). Our Avi-tag Biotinylated IL-1Rrp2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity
References
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 1 Receptor-like 2
Alternate Names
IL-1 R6, IL-1 Rrp2, IL-1RL2, IL-1Rrp2, IL1RL2, IL1Rrp2
Gene Symbol
IL1RL2
UniProt
Additional IL-1Rrp2/IL-1R6 Products
Product Documents for Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF
Product Specific Notices for Recombinant Human IL-1Rrp2/IL-1R6 Fc Avi-tag Protein, CF
For research use only
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