Recombinant Human IL-2 R beta Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10919-2B

R&D Systems, part of Bio-Techne
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10919-2B-050
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Key Product Details

Source

CHO

Accession #

P14784.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-2 R beta Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-2 R beta protein
Human IL-2 R beta
(Ala27-Asp239)
Accession # P14784.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

51 kDa

SDS-PAGE

62 - 68 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit Recombinant Human IL-15  (Catalog # 247-ILB) dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 1.75-14.0 μg/mL.

Scientific Data Images for Recombinant Human IL-2 R beta Fc Chimera Protein, CF

Recombinant Human IL‑2 R beta Fc Chimera Protein Bioactivity.

Recombinant Human IL‑2 R beta Fc Chimera (Catalog # 10919-2B) inhibits Recombinant Human IL-15 (247-ILB) dependent proliferation of MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 1.75-14.0 μg/mL.

Recombinant Human IL‑2 R beta Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑2 R beta Fc Chimera Protein (Catalog # 10919-2B) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-68 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

10919-2B
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2 R beta

Interleukin-2 receptor subunit beta (IL-2 RB), also known as high affinity IL-2 receptor subunit beta, interleukin-15 receptor subunit beta, p70-75 (p75), and CD122, is a member of the cytokine receptor superfamily that plays a role in T cell-mediated immune responses (1, 3). Human IL-2 RB consists of an extracellular domain (ECD) containing a fibronectin type III domain and a WSXWS motif, a type I transmembrane domain, and a cytoplasmic tail. Within the ECD, mature human IL-2 RB shares 58% and 61% amino acid sequence identity with mouse and rat IL-2 RB, respectively. A soluble IL-2 RB (sIL-2 RB) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 RB (5). Functional IL-2 receptors can exist in two affinity states on cell surfaces: the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains (2, 3). Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling (4). At present, the function of IL-2 sR beta is unclear. Recombinant human sIL-2RB binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, sIL-2 RB binds IL-15 with sufficient affinity to neutralize IL-15 biological activities. 

References

  1. Jia, Z. et al. (2019) Int. Immunpharmacol. 74:105716.
  2. Zhou, X. et al. (2019) Fish & Shellfish Immunol. 93:641.
  3. Zhang, Z. et al. (2019) J. Exp. Med. 216:1311.
  4. Fernandez, I. et al. (2019) J. Exp. Med. 216:1255.
  5. Honda, M. et al. (1990) J. Immunol. 145:4131.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Gene Symbol

IL2RB

UniProt

P14784.1

Additional IL-2 R beta Products

Product Documents for Recombinant Human IL-2 R beta Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human IL-2 R beta Fc Chimera Protein, CF

For research use only

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