Recombinant Human IL-2 R beta His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10459

Biotinylated
R&D Systems, part of Bio-Techne
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AVI10459-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P14784.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-2 R beta Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-2 R beta protein
Human IL-2 R beta
(Ala27-Asp239)
Accession # P14784.1		 6-His tag  Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

28 kDa

SDS-PAGE

36-45 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human IL-2 R beta His-tag Avi-tag (Catalog # AVI10459) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human IL-15  (Catalog # 247-ILB) binds with an ED50 of 1-6 ng/mL.

Scientific Data Images for Recombinant Human IL-2 R beta His-tag Avi-tag Protein, CF

Recombinant Human IL-2 R beta His-tag Avi-tag Protein Binding Activity

Recombinant Human IL-2 R beta His-tag Avi-tag Protein Binding Activity

When Biotinylated Recombinant Human IL-2 R beta His-tag Avi-tag (AVI10459) is immobilized at 0.25 µg/mL (100 µL/well), Recombinant Human IL-15 (247-ILB) binds with an ED50 of 1-6 ng/mL.
Recombinant Human IL-2 R beta His-tag Avi-tag Protein SDS-PAGE

Recombinant Human IL-2 R beta His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human IL-2 R beta His-tag Avi-tag (AVI10459) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 36-45 kDa.

Formulation, Preparation and Storage

AVI10459
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2 R beta

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities. Our Avi-tag Biotinylated Recombinant Human IL‑2 R beta features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Long Name

Interleukin 2 Receptor beta

Alternate Names

CD122, IL-15 R beta, IL-2Rb, IL2R beta, IL2RB

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Gene Symbol

IL2RB

UniProt

P14784.1

Additional IL-2 R beta Products

Product Documents for Recombinant Human IL-2 R beta His-tag Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human IL-2 R beta His-tag Avi-tag Protein, CF

For research use only

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