Recombinant Human IL-32 alpha Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3040-IL
Key Product Details
Product Specifications
Source
E. coli-derived human IL-32 alpha protein
Cys2-Lys131
Cys2-Lys131
Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys2
Predicted Molecular Mass
14.9 kDa
SDS-PAGE
18.8 kDa, reducing conditions
Activity
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cellsunder serum free conditions in the presence of muramyl dipeptide (MDP). Netea, M.G. et al. (2005) Proc. Nat. Acad. Sci. 102:16309.
The ED50 for this effect is 2-12 μg/mL.
The ED50 for this effect is 2-12 μg/mL.
Formulation, Preparation and Storage
3040-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and DTT. |
| Reconstitution |
Reconstitute at 200 μg/mL in PBS.
|
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-32 alpha
CXCL8/IL-8 (5-7). The longest of several IL-32 splicing variants is the 20 ‑ 25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or delta at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13 ‑ 15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).
References
- Netea, M.G. et al. (2006) PloS Med. 3:e277.
- Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. 106:3883.
- Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
- Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
- Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
- Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. 102:16309.
- Hong, J. et al. (2010) Cytokine 49:171.
- Kim, S.-H. et al. (2005) Immunity 22:131.
- Dahl, C.A. et al. (1992) J. Immunol. 148:597.
- Choi, J.-D. et al. (2009) Immunology 126:535.
- Novick, D. et al. (2006) Proc. Natl. Acad. Sci. 103:3316.
- Kim, S. et al. (2008) BMB Rep. 41:814.
- Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
- Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. 103:3298.
- Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
- Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
- Nold, M.F. et al. (2008) J. Immunol. 181:557.
Long Name
Interleukin 32 alpha
Alternate Names
IL32 alpha
Entrez Gene IDs
9235 (Human)
Gene Symbol
IL32
UniProt
Additional IL-32 alpha Products
Product Documents for Recombinant Human IL-32 alpha Protein, CF
Product Specific Notices for Recombinant Human IL-32 alpha Protein, CF
For research use only
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