Recombinant Human IL-33 Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT3625

Biotinylated
R&D Systems, part of Bio-Techne
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BT3625-025
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Key Product Details

Source

E. coli

Accession #

O95760.1

Structure / Form

Biotinylated via amines

Conjugate

Biotin

Applications

Bioactivity

View all IL-33 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-33 protein
Ser112-Thr270

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser112

Predicted Molecular Mass

18 kDa

SDS-PAGE

18-22 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ST2/IL-33R Fc Chimera (Catalog # 523-ST) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-33 (Catalog # BT3625) binds with an ED50 of 0.900-9.00 ng/mL.

Scientific Data Images for Recombinant Human IL-33 Biotinylated Protein, CF

Recombinant Human IL-33 Biotinylated Protein Binding Activity.

When Recombinant Human ST2/IL-33R Fc Chimera Protein (523-ST) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL‑33 Protein (Catalog # BT3625) binds with an ED50 of 0.900-9.00 ng/mL.

Recombinant Human IL-33 Biotinylated Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-33 Biotinylated Protein (Catalog # BT3625) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Formulation, Preparation and Storage

BT3625
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a 30-kDa proinflammatory protein that was identified based on sequence and structural homology with IL‑1 family cytokines (1-3). IL‑33 is constitutively expressed in smooth muscle and airway epithelia and is up-regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL‑1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL‑1, IL‑33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full-length IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain. By immunofluorescence, full-length IL‑33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL‑33 and ST2L with IL‑1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion (3). In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Mature human IL‑33 shares 52‑58% aa sequence identity with mouse and rat IL‑33 and shares less than 20% aa sequence identity with other IL‑1 family proteins.

References

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse)

Gene Symbol

IL33

UniProt

O95760.1

Additional IL-33 Products

Product Documents for Recombinant Human IL-33 Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human IL-33 Biotinylated Protein, CF

For research use only

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