Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein
R&D Systems, part of Bio-Techne | Catalog # 6834-ILB
Key Product Details
Product Specifications
Source
E. coli-derived human IL-36 beta/IL-1F8 protein
Arg5-Glu157
Arg5-Glu157
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg5
Predicted Molecular Mass
17 kDa
SDS-PAGE
17 kDa, reducing conditions
Activity
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells.
The ED50 for this effect is 0.8-4.8 ng/mL.
The ED50 for this effect is 0.8-4.8 ng/mL.
Scientific Data Images for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein
Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein Bioactivity
Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 6834-ILB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 6834-ILB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-36 beta/IL-1F8
IL-18, IL-36Ra/IL-1F5, IL-36 alpha/IL-1F6, IL-37/IL-1F7, IL-36 gamma/IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12 beta-stranded beta-trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta1 lacks four of the conserved beta-strands common to the IL-1 family (3). Human IL-36 beta2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).
References
- Garlanda, C. et al. (2013) Immunity 39:1003.
- Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Johnston, A. et al. (2011) J. Immunol. 186:2613.
- Wang, P. et al. (2005) Cytokine 29:245.
- Vigne, S. et al. (2012) Blood 120:3478.
- Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
- Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
- Foster, A.M. et al. (2014) J. Immunol. 192:6053.
- Vigne, S. et al. (2011) Blood 118:5813.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
- Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Alternate Names
FIL1 eta, IL-1 eta, IL-1H2, IL1F8, IL36 beta, IL36B
Gene Symbol
IL36B
UniProt
Additional IL-36 beta/IL-1F8 Products
Product Documents for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein
Product Specific Notices for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein
For research use only
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