Recombinant Human IL-37/IL-1F7 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1975-IL

R&D Systems, part of Bio-Techne
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1975-IL-025
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Key Product Details

Source

E. coli

Accession #

NP_775297

Conjugate

Unconjugated

Applications

Binding Activity

View all IL-37/IL-1F7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-37/IL-1F7 protein
Lys27-Asp192, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

18.6 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human (rh) IL‑18 R alpha/IL‑1 R5 Fc Chimera (Catalog # 816-LR) is immobilized at 2 μg/mL (100 μL/well), the concentration of rhIL-37/IL-1F7 Chimera that produces 50% optimal binding response is 20-120 ng/mL.

Formulation, Preparation and Storage

1975-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-37/IL-1F7

Human Interleukin-1 family member 7 (IL-1F7), also named FIL-1 zeta, IL-37, IL-1H4, IL-1HL and IL-1RP1, is an anti-inflammatory member of the IL-1 cytokine family (1-3). Alternative splicing generates multiple isoforms (IL-37a through e) with deletions in the N-terminal region of the molecule (4-7). The longest IL-1F7 transcript encodes IL-37b, which shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta, and IL-1ra, respectively. Mouse IL-37 has not been reported, but human IL-37b is active on mouse cells. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide. IL-37b is up-regulated by inflammatory stimuli in peripheral blood mononuclear cells (8). Experimental over-expression of IL-37b in vivo limits the inflammatory response and protects mice from colitis and LPS-induced shock (8, 9). Both unprocessed and mature IL-37b can form homodimers in solution (8, 10). Although IL-37b will bind to IL-18 R alpha with low affinity, this has no effect on IL-18 receptor activity (7, 10, 11). Alternatively, IL-37b will also bind to IL-18 BP, generating a complex that interacts with IL-18 R beta. This has the effect of attenuating IL-18 activity via the IL-18 receptor (11). IL-37b can also function intracellularly. Following LPS-induced cleavage of its propeptide, IL-37b associates with Smad3 and translocates to the nucleus. This results in a reduction of pro-inflammatory cytokine secretion (12).

References

  1. Dinarello, C.A. and P. Bufler (2013) Sem. Immunol. 25:466.
  2. Boraschi, D. et al. (2011) Eur. Cytokine Netw. 22:127.
  3. Ding, V.A. et al. (2016) Med. Oncol. 33:68.
  4. Smith, D.E. et al. (2000) J. Biol Chem 275:1169.
  5. Kumar S. et al. (2000) J. Biol Chem 275:10308.
  6. Busfield S.J. et al. (2000) Genomics 66:213.
  7. Pan, G. et al. (2000) Cytokine 13:1.
  8. Nold, M.F. et al. (2010) Nat. Immunol. 11:1014.
  9. McNamee, E.N. et al. (2011) Proc. Natl. Acad. Sci. USA 108:16711.
  10. Kumar S. et al. (2002) Cytokine 18:61.
  11. Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
  12. Sharma, S. et al. (2008) J. Immunol. 180:5477.

Long Name

Interleukin 37

Alternate Names

FIL1 zeta, IL-1F7, IL37

Entrez Gene IDs

27178 (Human)

Gene Symbol

IL37

UniProt

NP_775297 (Human)

Additional IL-37/IL-1F7 Products

Product Documents for Recombinant Human IL-37/IL-1F7 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-37/IL-1F7 Protein, CF

For research use only

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