Recombinant Human IL-3R alpha Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11140

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P26951.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-3R alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-3R alpha protein
Human IL-3RA
(Thr19-Arg305)
Accession # P26951.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.50 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr19 and Lys20

Predicted Molecular Mass

61 kDa

SDS-PAGE

83-93 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IL-3 (Catalog # 203-IL) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag (Catalog # AVI11140) binds with an ED50 of 3.00-45.0 ng/mL.

Scientific Data Images for Recombinant Human IL-3R alpha Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human IL-3 (203-IL) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag Protein (Catalog # AVI11140) binds with an ED50 of 3.00-45.0 ng/mL.

Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL-3R alpha/CD123 Fc Chimera Avi-tag Protein (Catalog # AVI11140) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-93 kDa and 170-190 kDa, respectively.

Formulation, Preparation and Storage

AVI11140
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R alpha

IL-3 receptor chain alpha (IL‑3R alpha) also known as CD123, is a member of the type 1 cytokine receptor family of proteins expressed mainly by activated T cells or mast cells (1-3). IL-3R alpha is a 60-70 kDa receptor component that binds IL-3 with low affinity and forms one-half of the IL-3 receptor complex. IL-3R alpha associates with the 130-140 kDa non-ligand binding IL-3R beta for high-affinity binding to IL-3 to form the heterodimeric receptor complex (4). IL-3R alpha consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain (3). Within the mature ECD, human IL-3R alpha shares 30% and 31% amino acid sequence identity with mouse and rat IL-3R alpha, respectively.  An isoform lacking the N-terminal domain of ECD has been identified in mouse and human (5, 6). IL‑3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage‑committed cells (7). IL-3R alpha is expressed on multiple cell types, including endothelial cells, monocytes, eosinophils, basophils plus mast cells, and plasmacytoid CD4+ T cells (8). IL-3R alpha is over-expressed in acute myeloid leukemia and this is associated with a poor prognosis in AML (9). Our Avi-tag Biotinylated human IL-3R alpha Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
  2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  3. Broughton, S.E. et al. (2014) Acta Crystallogr F Struc Biol Commun. 70:358.
  4. Broughton, S.E. et al. (2014) Cell Reports. 8:410.
  5. Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
  6. Hamming, O.J. et al. (2012) J Biol Chem. 287:9454.
  7. Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.
  8. Michalska, A. et al. (2020) Postepy Dermatol Alergol. 37:299.
  9. Testa, U. et al. (2002) Blood 100:2980.

Long Name

Interleukin 3 Receptor alpha

Alternate Names

CD123, IL-3 R alpha, IL-3Ra, IL3R alpha, IL3RA

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Gene Symbol

IL3RA

UniProt

P26951.1

Additional IL-3R alpha Products

Product Documents for Recombinant Human IL-3R alpha Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-3R alpha Fc Chimera Avi-tag Protein, CF

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