Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9949

R&D Systems, part of Bio-Techne
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AVI9949-050
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL1RAPL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL1RAPL1 protein
Human IL-1RAPL1
(Leu19-Thr357)
Accession # Q9NZN1.2		 GGIEGRMD
 Human IgG1
(Pro100-Lys330)		 Avitag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu19

Predicted Molecular Mass

67 kDa

SDS-PAGE

80-90 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag (Catalog # AVI9949) binds Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) with an ED50 of 1.50-20.0 ng/mL.

Scientific Data Images for Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein (Catalog # AVI9949) binds Recombinant Human PTPRD Fc Chimera (9995-PR) with an ED50 of 1.50-20.0 ng/mL.

Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein (Catalog # AVI9949) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-90 kDa and 160-180 kDa, respectively.

Formulation, Preparation and Storage

AVI9949
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL1RAPL1

Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), also known as Oligophenin-4 (OPHN4) and three immunoglobulin domain containing IL-1 receptor-related 2 (TIGIRR-2) (1), is a member of the IL-1 receptor superfamily. IL1RAPL1 is a single pass type I membrane protein which contains an N-terminal signal peptide (aa 1-18 ), three extracellular immunoglobulin-like domains (aa 19-350), a transmembrane domain (aa 358-378 ), an intracellular Toll/IL-1R domain (aa 403-562), and a long C-terminal tail which interacts with multiple signaling molecules (aa 549-644 ) (2). High expression levels of IL1RAPL1 was found in post-natal hippocampus, and its expression is upregulated by neuronal activity (3). The extracellular domain of IL1RAPL1 can mediate synapse formation through trans-synaptic interaction with PTPRD (4, 5). In neurons, IL1RAPL1 interacts with PSD-95, a major scaffolding protein of excitatory synapses, and modulates its synaptic localization by regulating JNK activity and PSD-95 phosphorylation (3).  Mutation or deletion of IL1RAPL1 gene is associated with non-syndromic intellectual disability and autism spectrum disorder (5). Human IL1RAPL1 shares 98% and 99% aa sequence identity with mouse and rat IL1RAPL1, respectively. Our Avi-tag Biotinylated human IL1RAPL1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
  2. Bahi, N. et al. (2003) Hum. Mol. Gen. 12:1415.
  3. Pavlowsky, A. et al. (2010) Curr. Biol. 20:103.
  4. Yoshida, T. et al. (2011) J. Neurosci. 31:13485.
  5. Ramos-Brossier, M. et al. (2015) Hum Mol Genet. 24:1106.

Long Name

Interleukin 1 Receptor Accessory Protein-like 1

Alternate Names

IL-1R9, IL-1RAPL1, TIGIRR-2

Entrez Gene IDs

11141 (Human); 331461 (Mouse); 317553 (Rat)

Gene Symbol

IL1RAPL1

Additional IL1RAPL1 Products

Product Documents for Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein, CF

For research use only

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