Recombinant Human Kallikrein 6 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5164-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Kallikrein 6/Neurosin protein
Glu17-Lys244, with a C-terminal 10-His tag
Glu17-Lys244, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu17
Predicted Molecular Mass
26 kDa
SDS-PAGE
35 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014).
The specific activity is >1300 pmol/min/µg, as measured under the described conditions.
The specific activity is >1300 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
5164-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Sodium Citrate. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Kallikrein 6/Neurosin
Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members (1, 2). The genes of this family aligned in tandem on chromosome 19q13.4 represent the largest contiguous group of proteases within the human genome. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung (2, 3). Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes (1 - 3). Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The level of hKLK6 is reduced in brain extracts of Alzheimer patients and increased in serum of patients with ovarian cancer.
References
- Emami, N. and E.P. Diamandis (2007) Clin Chim Acta 381:78.
- Borgoño, C.A. and E.P. Diamandis (2004) Nat. Rev. Cancer, 4:876.
- Paliouras, M. et al. (2007) Cancer Lett. 249:61.
Alternate Names
KLK6, Neurosin, Protease M, PRSS18, PRSS9, SP59, Zyme
Entrez Gene IDs
5653 (Human)
Gene Symbol
KLK6
UniProt
Additional Kallikrein 6/Neurosin Products
Product Documents for Recombinant Human Kallikrein 6 Protein, CF
Product Specific Notices for Recombinant Human Kallikrein 6 Protein, CF
For research use only
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