Recombinant Human Klotho His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10308-KL

Mucin Stalk Chimera
R&D Systems, part of Bio-Techne
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10308-KL-050
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Key Product Details

Source

CHO

Accession #

Q9UEF7 (Klotho) & P78423 (Fractalkine Mucin-like Stalk)

Conjugate

Unconjugated

Applications

Bioactivity

View all Klotho Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Klotho protein
Human Klotho
(Glu34-His549)
Accession # Q9UEF7		 Human Fractalkine Mucin-like Stalk
(Phe103-Thr338)
Accession # P78423		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu34

Predicted Molecular Mass

84 kDa

SDS-PAGE

110-140 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FGF-23 (Catalog # 2604-FG) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human Klotho Mucin Stalk Chimera His-tag (Catalog # 10308-KL) binds with an ED50 of 0.2-1.8 μg/mL.

Scientific Data Images for Recombinant Human Klotho His-tag Protein, CF

Recombinant Human Klotho His-tag Protein Binding Activity

Recombinant Human Klotho His-tag Protein Binding Activity

When Recombinant Human FGF-23 (Catalog # 2604-FG) is immobilized at 5 µg/mL, 100 µL/well, Recombinant Human Klotho Mucin Stalk Chimera His-tag (Catalog # 10308-KL) binds with an ED50 of 0.2-1.8 µg/mL.
Recombinant Human Klotho His-tag Protein SDS-PAGE

Recombinant Human Klotho His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Klotho Mucin Stalk Chimera His-tag (Catalog # 10308-KL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-140 kDa.

Formulation, Preparation and Storage

10308-KL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Klotho

Klotho, also called alpha-Klotho ( alpha-Klotho), is the founding member that along with beta-Klotho and gamma-Klotho, form the Klotho family within the glycosidase-1 superfamily (1, 2). alpha-Klotho is a type I transmembrane protein consisting of a large extracellular domain (ECD) containing glycosidase-like domains (KL1 and KL2), a single transmembrane domain and a short intracellular domain. Alternative mRNA splicing of the ECD of alpha-Klotho results in a circulating protein known as soluble alpha-Klotho (s-Klotho), which has been detected in both humans and mice (3, 4). In addition to the s-Klotho form, a 130 kDa form found in plasma and cerebrospinal fluid and a prominent intracellular 120 kDa form of alpha-Klotho have also been identified (3, 4). The mature ECD of full length human alpha-Klotho shares 87% and 90% identity with mouse and rat alpha-Klotho, respectively. Due to highly conserved sequences between alpha-Klotho forms, it is difficult to differentiate s-klotho from the other short forms in vivo (5). Although alpha-Klotho was identified ~20 years ago, its function remains incompletely understood. alpha-Klotho shows weak glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (10). alpha-Klotho acts as a cofactor for interaction of FGF-23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D) (7). s-Klotho functions as a hormonal factor and is involved in anti-aging, anti-oxidation, modulation of ion-transport, and Wnt signaling (8). Both alpha-Klotho and beta-Klotho are cofactors for FGF19 binding (9). The phenotype of alpha-Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). alpha-Klotho deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2-7). Conversely, excess alpha-Klotho extends lifespan (6).

References

  1. Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
  2. Kuro-o, M. et al. (1997) Nature 390:45.
  3. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
  4. Imura, A. et al. (2004) FEBS Lett. 565:143.
  5. Yuechi, X. et al. (2015) Endocr Rev. 36:174.
  6. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  7. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
  8. Hu, M. et al. (2012) Adv Exp Med Biol. 728:126.
  9. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  10. Chang, Q. et al. (2005) Science 310:490.

Alternate Names

KL

Entrez Gene IDs

9365 (Human); 16591 (Mouse)

Gene Symbol

KL

UniProt

Q9UEF7 (Klotho) & P78423 (Fractalkine Mucin-like Stalk)

Additional Klotho Products

Product Documents for Recombinant Human Klotho His-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Klotho His-tag Protein, CF

For research use only

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