Recombinant Human Kynurenine 3-Monooxygenase/KMO Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8050-KM

R&D Systems, part of Bio-Techne
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8050-KM-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

O15229

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Kynurenine 3-Monooxygenase/KMO Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Kynurenine 3-Monooxygenase/KMO protein
Asp2-Leu441, with an N-terminal Met and 6-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Inconclusive results, intact N-terminus verified by anti-poly-His Western

Predicted Molecular Mass

51 kDa

SDS-PAGE

40-45 kDa, reducing conditions

Activity

Measured by the consumption of NADPH by hydroxylation of L-kynurenine to 3-hydroxy-kynurenine.
The specific activity is >290 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8050-KM
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Kynurenine 3-Monooxygenase/KMO

Kynurenine 3-monooxygenase (KMO), also known as kynurenine 3-hydroxylase, is a part of the kynurenine pathway of tryptophan degradation (1). KMO catalyzes the NADPH- and flavin adenine dinucleotide (FAD)-dependent 3-hydroxylation of kynurenine to 3-hydroxykynurenine (3-HK). 3-HK is neurotoxic via the generation of hydrogen peroxide (2) and through the excitotoxic effects of its downstream metabolite quinolinic acid (3). The levels of 3-HK and quinolinic acid are increased in the brain with Alzheimer's disease and Huntington's disease (1). Inhibition of KMO was shown to reverse cognitive and motor deficits in mouse models of those diseases via an increase in neuroprotective kynurenic acid (4). KMO is found in the mitochondrial outer membrane of microglial cells in the brain and dendritic cells and macrophages in the periphery (1). This recombinant human KMO was expressed as a C-terminally truncated protein.

References

  1. Schwarcz, R. et al. (2012) Nat. Rev. Neurosci. 13:465.
  2. Okuda, S. et al. (1996) Proc. Natl. Acad. Sci. 93:12553.
  3. Stone, T.W. and M.N. Perkins. (1981) Eur. J. Pharmacol. 72:411.
  4. Zwilling, D. et al. (2011) Cell 145:863.

Alternate Names

KMO, Kynurenine 3Monooxygenase

Entrez Gene IDs

8564 (Human); 98256 (Mouse); 59113 (Rat)

Gene Symbol

KMO

UniProt

O15229

Additional Kynurenine 3-Monooxygenase/KMO Products

Product Documents for Recombinant Human Kynurenine 3-Monooxygenase/KMO Protein, CF

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Product Specific Notices for Recombinant Human Kynurenine 3-Monooxygenase/KMO Protein, CF

For research use only

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