Recombinant Human Lactate Dehydrogenase A/LDHA Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9158-HA

R&D Systems, part of Bio-Techne
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9158-HA-050
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Key Product Details

Source

E. coli

Accession #

P00338

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Lactate Dehydrogenase A/LDHA Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Lactate Dehydrogenase A/LDHA protein
Ala2-Phe332, with N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its ability to reduce pyruvate to lactate.
The specific activity is >95,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9158-HA
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Lactate Dehydrogenase A/LDHA

A hallmark of most cancer cells is an altered metabolism involving a shift to aerobic glycolysis with lactate production coupled with a higher uptake of glucose as the main source of energy. Lactate dehydrogenase (LDH) is key to this shift by catalyzing the formation of lactate by reducing pyruvate with NADH, which also generates NAD(+) that is essential for the continuity of glycolysis (1). LDHA is a key enzyme that controls the production of lactate in the glycolysis pathway. It is therefore an important control point in the system of cellular energy release. It's up regulation is common in many malignant tumors. Inhibiting LDH activity has an anti-proliferative effect on cancer cells (2). It may reverse the resistance of tumor cells to conventional chemo- and radiotherapy. Recent research has renewed interest in LDH as an anticancer drug target (3).The protein is found predominantly in muscle tissue. Mutations in LDHA have been linked to exertional myoglobinuria (4).

References

  1. Faloppi L. et al. (2016) Sci Rep. doi: 10.1038/srep24136.
  2. Ghosh, M. et al. (2016) Chem. Commun. 52:2401.
  3. Augoff, K. et al. (2015) Cancer Lett. 358:1.
  4. Maekawa M. et al. (1990). Biochem. Biophys. Res. Commun. 168:677.

Alternate Names

LDH1, LDHA, LDHM

Entrez Gene IDs

3939 (Human); 16828 (Mouse); 24533 (Rat)

Gene Symbol

LDHA

UniProt

P00338 (Human)

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Recombinant Human Lactate Dehydrogenase A/LDHA Protein, CF

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Product Specific Notices for Recombinant Human Lactate Dehydrogenase A/LDHA Protein, CF

For research use only

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