Recombinant Human LEDGF p52 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6044-LE

R&D Systems, part of Bio-Techne
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6044-LE-050
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Key Product Details

Source

E. coli

Accession #

NP_066967

Conjugate

Unconjugated

Applications

Bioactivity

View all LEDGF Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human LEDGF protein
Met1-Gln333

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

37.7 kDa

SDS-PAGE

52 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Able to significantly enhance neurite outgrowth when immobilized at 1.5-15 μg/mL on a nitrocellulose-coated microplate.

Formulation, Preparation and Storage

6044-LE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LEDGF

Lens epithelium-derived growth factor (LEDGF; also PC4 and SFRS1-interacting protein, PSIP, transcriptional co-activator p75/p52, and dense fine speckles 70 kDa protein) is a ubiquitously expressed, 75 kDa member of the hepatoma-derived growth factor (HDGF) family of proteins (1). Human LEDGF is 530 amino acids (aa) in length. LEDGF contains a PWWP domain (aa 1 - 64), a nuclear localization signal (aa 146 - 156), two coiled-coil regions (aa 306 - 334 and 371 - 395), and four helical regions (aa 347 - 362, 370 - 381, 394 - 403 and 410 - 425). In addition, there are multiple serine and threonine residues that are sites of potential phosphorylation. The protein is highly charged, with lysine, arginine, glutamate, and aspartate comprising 39% of the total residues (1). Two splicing variants produce a second isoform known as p52. p52 has an 8 aa substitution corresponding to aa 326 - 333 of LEDGF, and a deletion of the final 197 aa found in LEDGF. Human LEDGF shares 92% aa sequence identity with mouse and rat LEDGF. LEDGF functions as a transcriptional co-activator that is involved in neuroepithelial stem cell differentiation and neurogenesis (2). It is also a survival factor that is inducible by oxidative stress and protects cells from various stresses by upregulating stress-responsive genes (3 - 4). For example, TNF-alpha elevates the expression of LEDGF, which increases the expression of endogenous gamma-GHS-HS, the catalytic subunit of the regulating enzyme in GSH biosynthesis that constitutes a protective mechanism in limiting oxidative stress induced by inflammatory cytokines (3). LEDGF is also a major autoantigen in atopic dermatitis and other inflammatory conditions involving dysregulated apoptosis (5 - 7). Anti-LEDGF autoantibodies have been shown to have cytotoxic activity, suggesting their involvement in pathogenesis (6). In apoptotic cells, caspases cleave this protein at three sites within functionally important domains, generating two fragments of 65 and 58 kDa (6). Caspase cleavage abolishes the survival function of LEDGF and may generate variants of the protein that enhance apoptosis (6). LEDGF has also been shown to interact with lentiviral integrase (IN) proteins, including HIV-1 IN, directly, determining their nuclear localization and their tight association with nuclear DNA (8). This prevents proteosomal degradation of these proteins (8).

References

  1. Ge, H. et al. (1998) EMBO J. 17:6723.
  2. Chylack, L.T. et al. (2004) Exp. Eye Res. 79:941.
  3. Takamura, Y. et al. (2006) Am. J. Physiol. Cell Physiol. 290:554.
  4. Shinohara, T. et al. (2002) Prog. Retin. Eye Res. 21:341.
  5. Sagiura, K. et al. (2007) J. Invest. Dermatol. 127:75.
  6. Ganapathy, V. et al. (2003) Autoimmune Rev. 2:290.
  7. Chin, M.S. et al. (2006) J. Autoimmun. 27:17.
  8. Llano, M. et al. (2004) J. Biol. Chem. 279:55570.

Long Name

Lens Epithelium-derived Growth Factor

Alternate Names

CLL-associated antigen KW-7, DFS 70, PSIP1, Transcriptional coactivator p75/p52

Entrez Gene IDs

11168 (Human); 101739 (Mouse); 313323 (Rat)

Gene Symbol

PSIP1

UniProt

NP_066967

Additional LEDGF Products

Product Documents for Recombinant Human LEDGF p52 Protein, CF

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Product Specific Notices for Recombinant Human LEDGF p52 Protein, CF

For research use only

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