Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF
R&D Systems, part of Bio-Techne | Catalog # AVI8429
Biotinylated (HEK293-expressed)
Key Product Details
Learn more about Avi-tag Biotinylated Proteins
Source
HEK293
Accession #
Structure / Form
Biotinylated via Avi-tag
Conjugate
Biotin
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human LILRB2/CD85d/ILT4 protein
Human LILRB2/CD85d/ILT4 (Gln22-His458) Accession # Q8N423.4 |
6-His tag | Avi-tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gln22 inferred from enzymatic pyroglutamate treatment revealing Thr23
Predicted Molecular Mass
50 kDa
SDS-PAGE
65-75 kDa, under reducing conditions.
Activity
Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004) at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) that produces 50% of the binding response is 0.100-0.800 μg/mL.
When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004) at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) that produces 50% of the binding response is 0.100-0.800 μg/mL.
Scientific Data Images for Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF
Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein Binding Activity.
When Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag (Catalog # AVI8429) is captured on EvenCoat Streptavidin Coated Plates (CP004) at 2 μg/mL (100 µL/well), the concentration of Recombinant Human Angiopoietin-like 7 Protein (914-AN) that produces 50% of the optimal binding response is 0.100-0.800 μg/mL.Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein SDS-PAGE.
2 μg/lane of Biotinylated Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi-tag Protein (Catalog # AVI8429) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa.Formulation, Preparation and Storage
AVI8429
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution | Reconstitute at 500 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: LILRB2/CD85d/ILT4
References
- Kang, X. et al. (2016) Cell cycle 15:25.
- Dennis, G. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:13245.
- Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110:17957.
- Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:8856.
- Wu, J. and Horuzsko, A. (2009) Human immunology 70:353.
- Chen, H.M. et al. (2018) J. Clin. Invest. 128:5647.
Long Name
Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5
Alternate Names
CD85d, ILT4, LIR2, MIR10
Entrez Gene IDs
10288 (Human)
Gene Symbol
LILRB2
UniProt
Additional LILRB2/CD85d/ILT4 Products
Product Documents for Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF
Product Specific Notices for Recombinant Human LILRB2/CD85d/ILT4 His-tag Avi Protein, CF
For research use only
Loading...
Loading...
Loading...