Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1966-LM

R&D Systems, part of Bio-Techne
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1966-LM-050
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Key Product Details

Source

NS0

Accession #

Q14108

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all LIMPII/SR-B2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LIMPII/SR-B2 protein
Human LIMPII
(Arg27-Thr432)
Accession # Q14108		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg27

Predicted Molecular Mass

73 kDa (monomer)

SDS-PAGE

105-115 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Glucosylceramidase/GBA (Catalog # 7410-GHB) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LIMPII/SR-B2 Fc Chimera (Catalog # 1966-LM) binds with an ED50 of 100-800 ng/mL.

Formulation, Preparation and Storage

1966-LM
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1-3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

References

  1. Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. 
  2. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
  3. Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137.
  4. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
  5. Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.

Long Name

Lysosomal Integral Membrane Protein II

Alternate Names

CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2

Entrez Gene IDs

950 (Human); 12492 (Mouse)

Gene Symbol

SCARB2

UniProt

Q14108

Additional LIMPII/SR-B2 Products

Product Documents for Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

For research use only

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