Recombinant Human Lymphotoxin alpha2/beta1 Protein
R&D Systems, part of Bio-Techne | Catalog # 679-TX
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Noncovalently-linked heterotrimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Lymphotoxin protein
Leu35-Leu205 (LT alpha) & Leu54-Gly244 (LT beta), both with an N-terminal Met & Thr41 ‑ Leu205 (LT alpha)
Leu35-Leu205 (LT alpha) & Leu54-Gly244 (LT beta), both with an N-terminal Met & Thr41 ‑ Leu205 (LT alpha)
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met & Thr41 (LT alpha) & Met (LT beta)
Predicted Molecular Mass
18 kDa (LT alpha), 18.5 kDa ( LT beta)
SDS-PAGE
18-26 kDa, reducing conditions
Activity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells.
The ED50 for this effect is 0.5-3 ng/mL.
The ED50 for this effect is 0.5-3 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 679-TX
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 679-TX/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Lymphotoxin
Lymphotoxin-alpha (LT-alpha), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT-beta) are members of the TNF family. LT-alpha cDNA encodes a 205 amino acid secreted soluble protein with a 34 amino acid signal sequence. LT-beta cDNA encodes a 244 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 19 - 48), and an extracellular domain (residues 49 - 244). Secreted LT-alpha assembles as a soluble homotrimer, LT-alpha 3. In addition, secreted LT-alpha also complexes with the membrane associated LT-beta to generate two types of heterotrimers, LT-alpha 1/ beta2 and LT-alpha 2/ beta1 (1). The soluble LT-alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT-alpha 1/ beta2, binds only to the lymphotoxin beta receptor (LT betaR). LT-alpha 2/ beta1 is capable of binding LT betaR, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT-alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naive CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).
References
- Browning et al. (1993) Cell 72:847.
- Ettinger et al. (1996) PNAS 93:13102.
- Cuff et al. (1998) J. Immunol. 161:6853.
- Browning et al. (1997) J. Immunol. 159:3288.
- Gramaglia et al. (1999) J. Immunol. 162:1333.
Alternate Names
LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E
Gene Symbol
LTA
Additional Lymphotoxin Products
Product Documents for Recombinant Human Lymphotoxin alpha2/beta1 Protein
Product Specific Notices for Recombinant Human Lymphotoxin alpha2/beta1 Protein
For research use only
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