Recombinant Human MCAM/CD146 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9709-MA

R&D Systems, part of Bio-Techne
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9709-MA-050
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Key Product Details

Source

NS0

Accession #

AAA20922

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all MCAM/CD146 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MCAM/CD146 protein
Human MCAM/CD146
(Val24-Gly559)
Accession # AAA20922
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val24

Predicted Molecular Mass

87 kDa

SDS-PAGE

108-124 kDa, reducing condtions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human MCAM/CD146 Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, it binds Recombinant Human Galectin-3 (Catalog # 8259-GA). The concentration of Recombinant Human Galectin-3 that produces 50% of the optimal binding response is 0.25-1.25 μg/mL.

Scientific Data Images for Recombinant Human MCAM/CD146 Fc Chimera Protein, CF

Recombinant Human MCAM/CD146 Fc Chimera Protein Bioactivity

Recombinant Human MCAM/CD146 Fc Chimera Protein Bioactivity

When Recombinant Human MCAM/CD146 Fc Chimera is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human Galectin-3 (Catalog # 8259-GA) binds with an ED50 of 0.25-1.25 ug/mL.

Formulation, Preparation and Storage

9709-MA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCAM/CD146

Melanoma cell adhesion molecule (MCAM), also known as MUC18 or CD146, is a putative adhesion molecule that belongs to the immunoglobulin superfamily (IgSF) (1). MCAM is an approximately 113 kDa type I transmembrane glycoprotein that contains a 536 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane domain, and a 63 aa cytoplasmic domain. Two MCAM splice variants have been observed, which vary in the length of their cytoplasmic tail (2). The ECD of human MCAM contains 2 IgV and 3 IgC2 domains and shares 74% and 73% identity with mouse and rat, respectively. MCAM was originally described as a marker of malignant potential in melanoma and was reported to promote both invasion and metastasis (3). Since then, expression has been detected in endothelial cells throughout the body and MCAM has been shown to be involved multiple cellular events including adhesion, migration, proliferation and differentiation (4, 5). Additionally, MCAM has been implicated in recruitment of activated T cells to inflammatory sites and is up-regulated in various inflammatory diseases (5, 6). Inhibiting MCAM signaling has been suggested as a potential therapy for diverse diseases including inflammatory bowel disease and ovarian cancer (7, 8). As a cellular adhesion molecule (CAM), MCAM functions as a molecular mediator to facilitate inter-cellular interactions of homotypic or heterotypic cells, or to intervene in interactions of cell-to-extracellular matrix for responding to physiological signal (9). MCAM has also been shown to be the functional ligand for Galectin-3 on endothelial cell surfaces (9).

References

  1. Lehmann, J.M. et al. (1989) Proc Natl Acad Sci U.S.A. 86:9891.
  2. Alais, S. et al. (2001) J. Cell Sci. 114:1847.
  3. Shih, I.M. et al. (1994) Am J Pathol. 145:837.
  4. Schrage, A. et al. (2008) Histochem Cell Biol. 129:441.
  5. Wang Z and Yan X. (2013) Cancer Lett. 330:150.
  6. Bardin, N. et al. (2006) Inflamm Bowel Dis. 12:16.
  7. Xing, S. et al. (2014) Am J Pathol. 184:1604.
  8. Ma, X. et al. (2017) Oncol Lett. 13:1681.
  9. Colomb F, et al. (2017) J Biol Chem. 292:8381.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Gene Symbol

MCAM

UniProt

AAA20922

Additional MCAM/CD146 Products

Product Documents for Recombinant Human MCAM/CD146 Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human MCAM/CD146 Fc Chimera Protein, CF

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