Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10959-MS

C-Terminal
R&D Systems, part of Bio-Techne
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Key Product Details

Source

HEK293

Accession #

AAH09272.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Mesothelin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Mesothelin protein
Human Mesothelin
(Glu296-Gly580)
Accession # AAH09272.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu296

Predicted Molecular Mass

59 kDa

SDS-PAGE

65-80 kDa, reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CA125/MUC16   (Catalog # 5609-MU) is immobilized at 200 ng/mL (100 µL/well), Recombinant Human Mesothelin (aa 296-580) Fc Chimera binds with an ED50 of 1.00-6.00 ng/mL.

Scientific Data Images for Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF

Recombinant Human Mesothelin C-Terminal (aa 296‑580) Fc Chimera Protein Binding Activity.

When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 200 ng/mL (100 µL/well), Recombinant Human Mesothelin (aa 296-580) Fc Chimera Protein (Catalog # 10959-MS) binds with an ED50 of 1.00-6.00 ng/mL.

Recombinant Human Mesothelin C-Terminal (aa 296‑580) Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Mesothelin C-Terminal (aa 296‑580) Fc Chimera Protein (Catalog # 10959-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa and 130-160 kDa, respectively.

Formulation, Preparation and Storage

10959-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1.00 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mesothelin

Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and over-expressed in several human tumors (1, 2). The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40 kDa membrane-bound protein termed MSLN and a 31 kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell (3, 4). Cleaved, human MSLN remains attached to the cell surface via a GPI linkage and shares 58% amino acid sequence identity with mouse and rat MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor (3). Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 4). It is up‑regulated in mesotheliomas and a range of carcinomas and adenomas (5-7). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (7, 8). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (9). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (9-11).

References

  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  3. Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
  4. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
  5. Ordonez, N.G. (2003) Mod. Pathol. 16:192.
  6. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
  7. Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
  8. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
  9. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  10. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
  11. Robinson, B.W.S. et al. (2003) Lancet 362:1612.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

AAH09272.1

Additional Mesothelin Products

Product Documents for Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF

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Product Specific Notices for Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF

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