Recombinant Human Meteorin-like/METRNL Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9339-MN

R&D Systems, part of Bio-Techne
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9339-MN-050
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Key Product Details

Source

CHO

Accession #

Q641Q3

Conjugate

Unconjugated

Applications

Bioactivity

View all Meteorin-like/METRNL Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Meteorin-like/METRNL protein
Human Meteorin-like/METRNL
(Gln46-Asp311)
Accession # Q641Q3		 HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln46 inferred from enzymatic pyroglutamate treatment revealing Tyr47

Predicted Molecular Mass

32 kDa

Activity

Measured by its ability to induce STAT3 phosphorylation in E16-E18 embryonic rat cortical neurons.
5 μg/mL of Recombinant Human Meteorin-like/METRNL protein significantly induces phosphorylation of STAT3.

Scientific Data Images for Recombinant Human Meteorin-like/METRNL Protein, CF

Recombinant Human Meteorin-like/METRNL Protein Bioactivity

Recombinant Human Meteorin-like/METRNL Protein Bioactivity

Recombinant Human Meteorin-like/METRNL Induces Stat3 Phosphorylation in E16-E18 Rat Cortical Neurons. Treatments of E16-E18 rat cortical neurons with 1 μg/ml (1) and 5 μg/ml of Recombinant Human Meteorin-like/METRNL (2) (Catalog # 9339-MN) for 15 mintues results in Stat3 phosphorlation. Untreated E16-E18 rat cortical neurons (3).

Formulation, Preparation and Storage

9339-MN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 14 days, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL, Subfatin, Cometin and Interleukin 39) is a 311 amino acid (aa) protein that shows 58% similarity with Meteorin (in human). Human Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide with the secreted mature protein ~30 kDa (1). Mature human Meteorin‑like protein shares 79% and 80% aa sequence identity with mouse and rat METRNL, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide (1). Metrnl is expressed in various adult mouse tissues with the highest expression in muscle tissue, adipose tissue, and activated monocytes (2, 3). Its expression is induced in muscle after exercise and in adipose tissue upon cold exposure suggesting that this secreted protein is both a myokine and an adipokine (4). Neurotrophic activity has been ascribed to Metrnl as it enhances neurite outgrowth and neuroblast migration in vivo. This effect is mediated by the Jak-STAT3 and MEK-ERK pathways (5). Metrnl has also been shown to be indirectly involved in the "browning" of white fat to a more thermogenic "beige fat" through activation of the STAT6 pathway in adipose tissue macrophages, inducing the M2-regulatory cytokines IL-4 and IL-13 (6). Metrnl transgenic mice have demonstrated a role for Metrnl in insulin sensitization through up-regulation of PPAR gamma, a key transcription factor regulating adipocyte differentiation. PPAR gamma increases adipocyte differentiation, inhibits adipose inflammation, activates lipid metabolism, and sensitizes adipocytes to insulin (4).

References

  1. Accession # Q641Q3.
  2. Li, ZY et al. (2014) CNS Neurosci Ther. 20:344.
  3. Ushach, I et al. (2015) Clin Immunol. 156:119.
  4. Zheng, S. et al. (2016) Acta Pharmacol Sin. 37:571.
  5. Jorgensen, J.R. et al. (2012) Exp Neurol. 233:172.
  6. Rao, RR. et al. (2014) Cell. 157:1279.

Alternate Names

Meteorin like, METRNL

Entrez Gene IDs

284207 (Human); 210029 (Mouse); 316842 (Rat)

Gene Symbol

METRNL

UniProt

Q641Q3

Additional Meteorin-like/METRNL Products

Product Documents for Recombinant Human Meteorin-like/METRNL Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Meteorin-like/METRNL Protein, CF

For research use only

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