Recombinant Human Methionine Aminopeptidase 1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3537-ZN
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Methionine Aminopeptidase 1/METAP1 protein
His52-Phe386 & Lys53-Phe386, with a C-terminal 10-His tag
His52-Phe386 & Lys53-Phe386, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His52 & Lys53
Predicted Molecular Mass
38 kDa
SDS-PAGE
38 kDa, reducing conditions
Formulation, Preparation and Storage
3537-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Methionine Aminopeptidase 1/METAP1
The human METAP1 gene encodes Methionine Aminopeptidase 1, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). Inhibition of METAPs provides a novel strategy in developing anti-cancer drugs (3). The purified rhMETAP1 consists of amino acid residues 52/53 to 386. A previous report showed that the N-terminal 89 amino acid region was not essential for catalytic activity (4).
References
- Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
- Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
- Liu, S. et al. (1998) Science 282:1324.
- Addlagatta, A. et al. (2005) Biochemistry 44:14741.
Alternate Names
MAP1, MetAP1
Gene Symbol
METAP1
UniProt
Additional Methionine Aminopeptidase 1/METAP1 Products
Product Documents for Recombinant Human Methionine Aminopeptidase 1 Protein, CF
Product Specific Notices for Recombinant Human Methionine Aminopeptidase 1 Protein, CF
For research use only
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