Recombinant Human Methionine Aminopeptidase 1D Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4714-ZN

R&D Systems, part of Bio-Techne
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4714-ZN-020
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q6UB28

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Methionine Aminopeptidase 1D/MAP1D Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Methionine Aminopeptidase 1D/MAP1D protein
Arg44-Ala335, with an N-terminal Met and a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

34 kDa

SDS-PAGE

38 kDa, reducing conditions

Activity

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE).
The specific activity is >30 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

4714-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Methionine Aminopeptidase 1D/MAP1D

Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is over-expressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1-43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).

References

  1. Leszczyniecka, M. et al. (2006) Oncogene 25:3471.
  2. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta. 1477:157.
  3. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  4. Serero, A. et al. (2003) J. Biol. Chem. 278:52953.

Alternate Names

MAP1D, MetAP1D

Entrez Gene IDs

254042 (Human)

Gene Symbol

METAP1D

UniProt

Q6UB28

Additional Methionine Aminopeptidase 1D/MAP1D Products

Product Documents for Recombinant Human Methionine Aminopeptidase 1D Protein, CF

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Product Specific Notices for Recombinant Human Methionine Aminopeptidase 1D Protein, CF

For research use only

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