Recombinant Human Methionine Aminopeptidase 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3795-ZN

R&D Systems, part of Bio-Techne
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3795-ZN-020
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P50579

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Methionine Aminopeptidase 2/METAP2 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Methionine Aminopeptidase 2/METAP2 protein
Ala2-Tyr478, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

54 kDa

SDS-PAGE

66 kDa, reducing conditions

Activity

Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 9168-SE).
The specific activity is >15 pmol/min/µg, as measured using the described conditions.

Formulation, Preparation and Storage

3795-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Methionine Aminopeptidase 2/METAP2

The human METAP2 gene encodes Methionine Aminopeptidase 2, a member of the M24 family of metalloproteases. METAPs catalyze the removal of the initiator methionine residue from nascent peptides (1) and are essential for cell growth (2). METAP2 plays an important role in the development of different types of cancer (3) and has been a novel target for developing anti-cancer drugs.

References

  1. Lowther, W.T. and B.W. Matthews (2000) Biochim. Biophys. Acta 1477:157.
  2. Li, X. and Y.H. Chang (1995) Proc. Natl. Acad. Sci. USA 92:12357.
  3. Selvakumar, P. et al. (2006) Biochim. Biophys. Acta 1765:148.

Alternate Names

Amp2, METAP2, MNPEP, p67eIF2

Entrez Gene IDs

10988 (Human); 56307 (Mouse); 64370 (Rat)

Gene Symbol

METAP2

UniProt

P50579

Additional Methionine Aminopeptidase 2/METAP2 Products

Product Documents for Recombinant Human Methionine Aminopeptidase 2 Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human Methionine Aminopeptidase 2 Protein, CF

This product is covered by US Patent Nos. 5,885,820 and 5,888,796 licensed from Mediomics, LLC.

For research use only

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