Recombinant Human mGluR5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9529-UR

R&D Systems, part of Bio-Techne
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9529-UR-100
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Key Product Details

Source

NS0

Accession #

P41594

Structure / Form

Disuflide linked homodimer

Conjugate

Unconjugated

View all mGluR5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human mGluR5 protein
Ser19-Ser509, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser19

Predicted Molecular Mass

56 kDa

SDS-PAGE

61-71 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9529-UR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR5

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). Group I includes mGluR1 and mGluR5. This group has been shown to activate phospholipase C and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length. The 2-509 amino acids domain represents the ECD, with the apparent molecular mass approximately 64 kDa in SDS-PAGE under reducing conditions. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca++ sensor receptor (4). Over aa 2-509, human mGluR5 is 98% aa identical to mouse and rat mGluR5.

References

  1. Conn PJ, et al. (1997) Annu Rev Pharmacol Toxicol 37:205.
  2. Pin JP, et al. (1995) Neuropharmacology. 34:1.
  3. Niswender CM, et al. (2010) Annu Rev Pharmacol Toxicol 50:295.
  4. Doré AS, et al. (2014) Nature 511:557.

Long Name

Metabotropic Glutamate Receptor 5

Alternate Names

GPRC1E, GRM5, mGlu5

Entrez Gene IDs

2915 (Human); 108071 (Mouse); 24418 (Rat)

Gene Symbol

GRM5

UniProt

P41594

Additional mGluR5 Products

Product Documents for Recombinant Human mGluR5 Protein, CF

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Product Specific Notices for Recombinant Human mGluR5 Protein, CF

For research use only

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