Recombinant Human Mindin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11539-SP

R&D Systems, part of Bio-Techne
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11539-SP-050
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Key Product Details

Source

NS0

Accession #

AAH02707.1

Conjugate

Unconjugated

Applications

Bioactivity

View all Mindin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Mindin protein
Glu32-Val331, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu 32

Predicted Molecular Mass

34 kDa

SDS-PAGE

38-44 kDa, under reducing conditions

Activity

Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 0.200-2.40 μg/mL.

Scientific Data Images for Recombinant Human Mindin His-tag Protein, CF

Recombinant Human Mindin His-tag Protein Binding Activity.

Recombinant Human Mindin His-tag Protein (Catalog # 11539-SP) binds to fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 0.200-2.40 μg/mL.

Formulation, Preparation and Storage

11539-SP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. 
Reconstitution Reconstitute lyophilized material at 250 μg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mindin

Mindin, also known as Spondin-2 and DIL-1, is a member of the Mindin F-Spondin family of evolutionarily conserved, secreted extracellular matrix proteins. It is composed of an N-terminal F-spondin domain, which mediates integrin binding, and a C-terminal thrombospondin type I repeat, which binds to bacterial lipopolysaccharide (1).  The mature human protein shares 88% aa sequence identity with the mouse orthologs (2). Mindin is expressed in a variety of tissues in mice with the highest expression levels being detected in lung, spleen, heart, and lymph nodes (2). Mindin is essential for initiating innate and adaptive immune responses. It has been shown to be a pattern recognition receptor for bacterial pathogens and function as an opsonin for macrophage phagocytosis of bacteria (1, 2). Mindin also binds directly to influenza virus particles and is necessary for macrophage-dependent clearance of influenza viruses from the nasal cavity (3). In addition, Mindin serves as a ligand for leukocyte integrins (1). It is critical for T cell priming and recruitment of macrophages and neutrophils to sites of inflammation (4, 5). Mindin also regulates trafficking of eosinophils and granulocytes into the airspace and plays a role in the development of allergic airways disease (6, 7). Outside the immune system, Mindin has been shown to promote adhesion and outgrowth of hippocampal embryonic neurons, and to be an important mediator of brain ischemic injury and cardiac hypertrophy (8-11). Furthermore, it has been suggested that Mindin can serve as a marker for prostate and ovarian cancer, as well as diabetic nephropathy (12-14).

References

  1. Li, Y. et al. (2009) EMBO J. 28:286.
  2. He, Y.W. et al. (2004) Nat. Immunol. 5:88.
  3. Jia, W. et al. (2008) J. Immunol. 180:6255.
  4. Li, H. et al. (2006) EMBO J. 25:4097.
  5. Jia, W. et al. (2005) Blood 106:3854.
  6. Li, Z. et al. (2009) J. Leukoc. Biol. 85:124.
  7. Tighe, R.M. et al. (2011) J. Allergy Ther. 2011 (Suppl. 1). pii: 001.
  8. Feinstein, Y. et al. (1999) Development 126:3637.
  9. Yan, L. et al. (2011) Cardiovasc. Res. 92:85.
  10. Bian, Z.Y. et al. (2012) J. Mol. Med. (Berl.) 90:895.
  11. Wang, L. et al. (2013) Exp. Neurol. 247:506.
  12. Simon, I. et al. (2007) Gynecol. Oncol. 106:112.
  13. Murakoshi, M. et al. (2011) Exp. Diabetes Res. 2011:486305.
  14. Lucarelli, G. et al. (2013) J. Urol. 190:2271.

Alternate Names

DIL1, RG-1, SPON2, Spondin 2

Entrez Gene IDs

10417 (Human); 100689 (Mouse)

Gene Symbol

SPON2

UniProt

AAH02707.1 (Human)

Additional Mindin Products

Product Documents for Recombinant Human Mindin His-tag Protein, CF

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Product Specific Notices for Recombinant Human Mindin His-tag Protein, CF

For research use only

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