Recombinant Human MMP-14/MT1-MMP (NS0-expressed) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 918-MPN

R&D Systems, part of Bio-Techne
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918-MPN-010
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Key Product Details

Source

NS0

Accession #

P50281

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MMP-14/MT1-MMP Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MMP-14/MT1-MMP protein
Ala21-Ser538, with an Arg108Pro mutation and a C-terminal 6-His tag

Purity

>80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

60 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2 (Catalog # ES010).
The specific activity is >350 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

918-MPN
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl, CaCl2, Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: MMP-14/MT1-MMP

The first identified member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type 1 collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alphav (1). MMP‑14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion (2). Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, four hemopexin-like domains, a transmembrane domain, and a cytoplasmic tail (3). Recombinant human (rh) MMP-14, consists of the pro domain, catalytic domain, hinge region and hemopexin-like domains. Because it lacks the transmembrane domain, rhMMP-14 is secreted and purified as a soluble protein. The pro domain was engineered to contain an Arg108Pro mutation, which prevents activation by furin cleavage. Activation of rhMMP-14 occurs by treatment with rhTrypsin 3 as described in the Activity Assay Protocol.

References

  1. Seiki, M. (2003) Cancer Lett. 194:1.
  2. Friedl, P. and K. Wolf (2008) Cancer Res. 68:7247.
  3. Sato, H. et al. (1994) Nature 370:61.

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Entrez Gene IDs

4323 (Human)

Gene Symbol

MMP14

UniProt

P50281

Additional MMP-14/MT1-MMP Products

Product Documents for Recombinant Human MMP-14/MT1-MMP (NS0-expressed) Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MMP-14/MT1-MMP (NS0-expressed) Protein, CF

For research use only

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