Recombinant Human MOG Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8535-MO

R&D Systems, part of Bio-Techne
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8535-MO-050
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Key Product Details

Source

NS0

Accession #

Q16653

Conjugate

Unconjugated

Applications

Bioactivity

View all MOG Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MOG protein
Gly30-Gly154, with a substitution at Asp131Ala and a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly30

Predicted Molecular Mass

16 kDa

SDS-PAGE

20-30 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Human MOG, immobilized at 1 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.

Formulation, Preparation and Storage

8535-MO
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MOG

Myelin oligodendrocyte glycoprotein (MOG) is 28 kDa single-pass transmembrane glycoprotein that is a member of the Ig superfamily (1, 2). Human MOG is synthesized with a 29 amino acid (aa) signal sequence, a 125 aa extracellular domain (ECD) containing an Ig-like domain, a 21 aa transmembrane domain, and a 72 aa cytosolic fragment featuring a hydrophobic domain that associates with the cytoplasmic face of the plasma membrane. The ECD of mature human MOG shares 90% aa sequence identity with the ECD of both mouse and rat MOG. Unlike mouse and rat, human MOG has seven splice variants, including a soluble ECD isoform and multiple isoforms that have a truncated cytoplasmic domain (3, 4). Dimerization of MOG occurs via the extracellular Ig-like domain (5-8). MOG is expressed exclusively by oligodendrocytes in the central nervous system (CNS) and is localized to the outer layer of the myelin sheath as well as in the oligodendrocyte plasma membrane (9). MOG expression in the brain can be used as a temporal biomarker for myelin development. MOG is an important antigenic target for autoimmune diseases that mediate demyelination in the CNS (10). In vivo administration of exogenous MOG protein or peptide induces experimental autoimmune encephalomyelitis (EAE) in multiple animal species (11, 12). EAE is used as an animal model for multiple sclerosis and related CNS demyelinating diseases. MOG is thought to function as an adhesion molecule as well as a mediator of immune activation in the CNS (9, 13).

References

  1. Johns, T.G. and C.C. Bernard (1999) J. Neurochem. 72:1.
  2. Pham-Dinh, D. et al. (1993) Proc. Natl. Acad. Sci. U S A 90:7990.
  3. Hilton, A.A. et al. (1995) J. Neurochem. 65:309.
  4. Slavin, A.J. et al. (1997) Dev. Neurosci. 19:69.
  5. Abo, S. et al. (1993) Biochem. Mol. Biol. Int. 30:945.
  6. Amiguet, P. et al. (1992) J. Neurochem. 58:1676.
  7. Bettadapura, J. et al. (1998) J. Neurochem. 70:1593.
  8. Clements, C.S. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:11059.
  9. Reindl, M. et al. (2013) Nat. Rev. Neurol. 9:455.
  10. von Budingen, H.C. et al. (2004) Eur. J. Immunol. 34:2072.
  11. Rangachari, M. and V.K. Kuchroo (2013) J. Autoimmun. 45:31.
  12. Tompkins, S.M. et al. (2002) J. Immunol. 168:4173.
  13. Garcia-Vallejo, J.J. et al. (2014) J. Exp. Med. 211:1465.

Long Name

Myelin Oligodendrocyte Glycoprotein

Alternate Names

MGC26137, MOG Ig-AluB, MOGIG2, myelin oligodendrocyte glycoprotein, myelin-oligodendrocyte glycoprotein

Entrez Gene IDs

4340 (Human); 17441 (Mouse)

Gene Symbol

MOG

UniProt

Q16653

Additional MOG Products

Product Documents for Recombinant Human MOG Protein, CF

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Product Specific Notices for Recombinant Human MOG Protein, CF

For research use only

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