Recombinant Human/Mouse/Rat GDF-8/Myostatin GMP Protein, CF GMP
R&D Systems, part of Bio-Techne | Catalog # 788-GMP
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived GDF-8/Myostatin protein
Asp268-Ser376
Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.
Asp268-Ser376
Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.
Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp-Phe-Gly-Leu-Asp-(Cys)-Asp-Glu-His-Ser
Predicted Molecular Mass
12.4 kDa
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing conditions
24 kDa, non-reducing conditions
Activity
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
The ED50 for this effect is 2-10 ng/mL.
The ED50 for this effect is 2-10 ng/mL.
Host Cell Protein
< 5.0 ng per µg of protein when tested by ELISA.
Mycoplasma
Negative when tested in a ribosomal RNA hybridization assay.
Scientific Data Images for Recombinant Human/Mouse/Rat GDF-8/Myostatin GMP Protein, CF
Recombinant Human/Mouse/Rat GDF-8/Myostatin GMP Protein Bioactivity
GMP-grade Recombinant Human/Mouse/Rat GDF-8/Myostatin (Catalog # 788-GMP) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The ED50 for this effect is 2‑10 ng/mL.
Recombinant Human/Mouse/Rat GDF-8/Myostatin GMP Protein SDS-PAGE
1 μg/lane of GMP-grade Recombinant Human/Mouse/Rat GDF-8/Myostatin (Catalog # 788-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing the main bands at 12 kDa and 24 kDa, respectively.Formulation, Preparation and Storage
788-GMP
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: GDF-8/Myostatin
Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF‑ betas, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.
References
- Storm, E.E. et al. (1994) Nature 368:639.
- Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
- McPherron, A.C. et al. (1997) Nature 387:83.
- Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
- Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
Long Name
Growth Differentiation Factor 8
Alternate Names
GDF8, MSLHP, MSTN, Myostatin
Gene Symbol
MSTN
UniProt
Additional GDF-8/Myostatin Products
Product Documents for Recombinant Human/Mouse/Rat GDF-8/Myostatin GMP Protein, CF
Manufacturing Specifications
GMP ProteinsR&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.
R&D Systems' quality focus includes:
- Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
- Documented processes and QA control of documentation and process changes
- Personnel training programs
- Raw material testing and vendor qualification/monitoring
- Fully validated equipment, processes and test methods
- Equipment calibration schedules using a computerized calibration program
- Facility maintenance, safety programs and pest control
- Material review process for variances
- Monitoring of stability over product shelf-life
- N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
- Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP
- Host Cell Protein testing performed by ELISA
- Mycoplasma testing by ribosomal RNA hybridization assay
Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.
R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.
Product Specific Notices for Recombinant Human/Mouse/Rat GDF-8/Myostatin GMP Protein, CF
END USER TERMS OF USE OF PRODUCT
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For preclinical, or clinical ex vivo use
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