Recombinant Human MVK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9349-VK

R&D Systems, part of Bio-Techne
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9349-VK-050
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Key Product Details

Source

E. coli

Accession #

Q03426

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MVK Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human MVK protein
Met1-Leu396, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

43 kDa

SDS-PAGE

38-46 kDa, reducing conditions

Activity

Measured by its ability to transfer phosphate from ATP to mevalonic acid.
The specific activity is >2,400 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

9349-VK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: MVK

Mevalonate kinase converts (R)-mevalonate to (R)-5-phosphomevalonate using ATP as the donor substrate (1). It is a key early enzyme in isoprenoid and sterol synthesis and may be a regulatory site in cholesterol biosynthetic pathway (2). Deficiency of the enzyme results in mevalonic aciduria, hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash (3, 4, 5). The enzymatic activity of the recombinant human MVK is measured using a phosphatase-coupled method (6).

References

  1. Green, T.R. and Baisted, D.J. (1970) Anal. Biochem. 38:130.
  2. Redding-Johanson A.M., et al. (2011) Metab. Eng. 13:194.
  3. Hager, E.J. and Gibson, K.M. (2007) N. Engl. J. Med. 357:1871.
  4. Favier, L.A. and Schulert, G.S. (2016) Appl. Clin. Genet. 9:101.
  5. Stoffels, M. et al. (2014) Rheumatol. Int. 34:295.
  6. Wu, Z.L. (2011) PLoS ONE 6:e23172.

Long Name

Mevalonate Kinase

Alternate Names

LRBP, Mevalonic Aciduria, MVLK, POROK3

Entrez Gene IDs

4598 (Human); 17855 (Mouse); 81727 (Rat)

Gene Symbol

MVK

UniProt

Q03426

Additional MVK Products

Product Documents for Recombinant Human MVK Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human MVK Protein, CF

For research use only

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