Recombinant Human Myocilin His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3446-MY

R&D Systems, part of Bio-Techne
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3446-MY-050
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Key Product Details

Source

HEK293

Conjugate

Unconjugated

Applications

Bioactivity

View all Myocilin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Myocilin protein
Leu215-Met504, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu215

Predicted Molecular Mass

34 kDa

SDS-PAGE

28-35 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of AGS human Gastric Adenocarcinoma cells.
The ED50 for this effect is 0.3-3 μg/mL.

Scientific Data Images for Recombinant Human Myocilin His-tag Protein, CF

Recombinant Human Myocilin His-tag Protein Bioactivity

Recombinant Human Myocilin His-tag Protein Bioactivity

Immobilized Recombinant Human Myocilin supporst the adhesion of AGS human Gastric Adenocarcinoma cells. The ED50 for this effect is 0.3-3 μg/mL.
Recombinant Human Myocilin His-tag Protein SDS-PAGE

Recombinant Human Myocilin His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Myocilin His-tag (Catalog# 3446-MY) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 28-35 kDa.

Formulation, Preparation and Storage

3446-MY
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocilin

Myocilin is a secreted glycoprotein that belongs to the family of olfactomedin-related proteins (1). Mature human Myocilin is synthesized as a 472 amino acid (aa) precursor that can be cleaved into a 194 aa N-terminal fragment containing leucine zipper motifs within two coil–coil domains and a 335 aa C-terminal fragment containing an olfactomedin (OLF) domain (2). The human OLF-domain shares 87% aa sequence identity with the OLF-domain in mouse and rat. The Myocilin gene is expressed in the human eye compartments. When it is secreted, it can be detected in the cornea, trabecular meshwork, aqueous humor, iris, ciliary body, choroid sclera, retina and the axons of optic nerve ganglion cells (3). Mutations of the MYOC gene are associated with primary open angle glaucoma (POAG), which is a complex disorder with a major heritable component (4). Myocilin interacts with itself to form dimers or multimers (2, 5-8), flotilin-1, optimedin, fibronectin, and fibrilin-1 as well as hevin and SPARC (2, 8-10). Myocilin stimulates cell migration that involves the activation of intergrin focal adhesion kinase (FAK)-serin/threonine kinase (AKT) signaling pathway (11). Myocilin binds specifically to the Heparin II domain of fibronectin (12).

References

  1. Zeng, L.C. et al. (2005) FEBS Lett. 579:5443.
  2. Resch, Z.T. and Fautsch M.P. (2009) Exp Eye Res. 88:704.
  3. Karali, A. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:729.
  4. Ikezoe, T. et al. (2003) Int J Mol Med. 12:259.
  5. Aroca-Aguilar, J.D. et al. (2013) PLoS One 8:e54385.
  6. Tamm, E.R. (2002) Prog Retin Eye Res 21:395.
  7. Fautsch, M.P. et al. (2006) Exp Eye Res 82:1046.
  8. Aroca-Aguilar, J.D. et al. (2011) Invest Ophthalmol Vis Sci 52:179.
  9. Wentz-Hunter, K. et al. (2002) Invest Ophthalmol Vis Sci 43:176.
  10. Ueda, J. et al. (2000) J Histochem Cytochem 48:1321.
  11. Kwon, H.S. and Tomarev, S.I. (2011) J Cell Physiol 226:3392.
  12. Filla, M.S. et al. (2002) Invest Ophthalmol Visual Sci. 43:151.

Alternate Names

GLC1A, MYOC, TIGR

Entrez Gene IDs

4653 (Human)

Gene Symbol

MYOC

Additional Myocilin Products

Product Documents for Recombinant Human Myocilin His-tag Protein, CF

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Product Specific Notices for Recombinant Human Myocilin His-tag Protein, CF

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