Recombinant Human N-Acetylglucosaminyltransferase V/MGAT5 CF

N‑Acetylglucosaminyltransferase V (GnT‑V), also known as mannosylglycoprotein N‑acetyl-glucosaminyltransferase 5 (MGAT5), adds an N‑acetylglucosamine to the alpha1‑6‑linked core mannose of an N‑linked oligosaccharide in the Golgi apparatus (1). This reaction is the committing step for the biosynthesis of beta1‑6GlcNAc-branched arm in N‑glycans. The degree of N‑glycan branching has been shown to regulate cell proliferation and differentiation (2). An increase in the GnT-V activity and its glycan products is also known to positively correlate with the progression of invasive malignancies (3, 4). For example, ectopic expression of GnT‑V in epithelial cells results in morphological transformation and tumor growth in mice and overexpression in carcinoma cells has been shown to induce metastatic spread (3‑5).The enzymatic activity of recombinant human MGAT5 was determined using a phosphatase-coupled glycosyltransferase assay (6).