Recombinant Human Neogenin Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9699-NE

R&D Systems, part of Bio-Techne
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9699-NE-050
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Key Product Details

Source

HEK293

Accession #

Q92859-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Neogenin Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Neogenin protein
Human Neogenin
(Ala34-Met1104)
Accession # Q92859-1
 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala34

Predicted Molecular Mass

144 kDa 

SDS-PAGE

142-164 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Neogenin Fc Chimera is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse Netrin-1 (Catalog # 1109-N1) that produces 50% optimal binding response is 3-15 ng/mL.

Scientific Data Images for Recombinant Human Neogenin Fc Chimera Protein, CF

Recombinant Human Neogenin Fc Chimera Protein Binding Activity

Recombinant Human Neogenin Fc Chimera Protein Binding Activity

When Recombinant Human Neogenin Fc Chimera (Catalog # 9699-NE) is coated onto a microplate at 1 µg/mL, Recombinant Mouse Netrin-1 (Catalog # 1109-N1) binds with an ED50 of 3-18 ng/mL.

Formulation, Preparation and Storage

9699-NE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neogenin

Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2 type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the human protein shares 91% and 93% amino acid sequence identity with the mouse and rat orthologues, respectively (1). Four different isoforms are produced from alternative splicing of human NEO1. Neogenin is widely expressed in adult human tissues with the highest levels being observed in skeletal muscle and pancreas (1). It is also ubiquitously expressed in both neuronal and non-neuronal tissues of the developing mouse embryo (2). Neogenin is a multifunctional cell surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates Netrin-1 dependent attraction and RGM-A dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorigenesis and cancer cell invasiveness in brain and gastric cancers (12-14).

References

  1. Meyerhardt, J.A. et al. (1997) Oncogene 14:1129.
  2. Keeling, S.L. et al. (1997) Oncogene 15: 691.
  3. Hagihara, M. et al. (2011) J. Biol. Chem. 286: 5157.
  4. Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700.
  5. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  6. van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828.
  7. De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483.
  8. Krauss, R.S. et al. (2005) J. Cell. Sci. 118:2355.
  9. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  10. de Castro, F. (2003) News Physiol. Sci. 18:130.
  11. O' Leary, C.J. et al. (2015) Stem Cells 33:503.
  12. Milla, L.A. et al. (2014) Int. J. Cancer 134:21.
  13. Akino, T. et al. (2014) Cancer Res. 74: 3716.
  14. Kim, S.J. et al. (2014) Oncotarget 5:3386

Alternate Names

NEO, NEO1, NGN

Entrez Gene IDs

4756 (Human); 18007 (Mouse)

Gene Symbol

NEO1

UniProt

Q92859-1

Additional Neogenin Products

Product Documents for Recombinant Human Neogenin Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Neogenin Fc Chimera Protein, CF

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