Recombinant Human Nephronectin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9560-NP

R&D Systems, part of Bio-Techne
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9560-NP-050
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Key Product Details

Source

CHO

Accession #

Q6UXI9-1

Conjugate

Unconjugated

Applications

Bioactivity

View all Nephronectin Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Nephronectin protein
Glu20-Arg565, with Val144Ile, Ile234Val, Asp377Asn substitutions and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu20

Predicted Molecular Mass

61 kDa

SDS-PAGE

72-95 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells.
The ED50 for this effect is 20-120 ng/mL.

Scientific Data Images for Recombinant Human Nephronectin Protein, CF

Recombinant Human Nephronectin Protein Bioactivity

Recombinant Human Nephronectin Protein Bioactivity

Immobilized Recombinant Human Nephronectin supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 20-120 ng/mL.

Formulation, Preparation and Storage

9560-NP
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, EDTA and CHAPS.
Reconstitution
Reconstitute at 200 μg/mL in water.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephronectin

Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70-90 kDa extracellular matrix protein that is a ligand for integrin alpha 8 beta 1 (1, 2). The 565 amino acid (aa) human Nephronectin contains a 19 aa signal sequence, a matrilin-type vWA domain, three calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain (1, 3, 4). These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2, 5). Six human Nephronectin isoforms were identified with alternative splicing. Mature human Nephronectin shows 88% aa identity with both mouse and rat Nephronectin. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down-regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalized, and forms an in-vivo complex with integrin alpha 8 beta 1 (2). Since deletion of integrin alpha 8 beta 1 results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha 8 beta 1 ligand during Nephrogenesis (2, 6).

References

  1. Morimura, N. et al. (2001) J. Biol. Chem. 276:42172.
  2. Brandenberger, R. et al. (2001) J. Cell Biol. 154:447.
  3. Sato, Y. et al. (2009) J. Biol. Chem. 284:14524.
  4. Patra, C. et al. (2012) Biomaterials. 33:4327.
  5. Yamanda, A. and R. Kamijo (2016) J. Dent. & Oral Disord. 2:1004.
  6. Miner, J. H. (2001) J. Cell Biol. 154:257.

Alternate Names

EGFL6L, Nctn, NPNT, POEM

Entrez Gene IDs

255743 (Human); 114249 (Mouse)

Gene Symbol

NPNT

UniProt

Q6UXI9-1

Additional Nephronectin Products

Product Documents for Recombinant Human Nephronectin Protein, CF

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Product Specific Notices for Recombinant Human Nephronectin Protein, CF

For research use only

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