Recombinant Human Neprilysin-2/MMEL1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2340-ZN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Neprilysin-2/MMEL1 protein
Gly69-Trp770, with an N-terminal 6-His tag
Gly69-Trp770, with an N-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His
Predicted Molecular Mass
82 kDa
SDS-PAGE
99 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >250 pmol/min/µg, as measured under the described conditions.
The specific activity is >250 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2340-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and ZnCl2. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Neprilysin-2/MMEL1
Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.
References
- Turner, A.J. et al. (2001) BioEssays 23:261.
- Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.
Alternate Names
MMEL1, MMEL2, Neprilysin2, NL2, SEP
Entrez Gene IDs
79258 (Human)
Gene Symbol
MMEL1
UniProt
Additional Neprilysin-2/MMEL1 Products
Product Documents for Recombinant Human Neprilysin-2/MMEL1 Protein, CF
Product Specific Notices for Recombinant Human Neprilysin-2/MMEL1 Protein, CF
For research use only
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