Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5268-NX

R&D Systems, part of Bio-Techne
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5268-NX-050
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Key Product Details

Source

NS0

Accession #

NP_620072

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all Neurexin 1 beta/NXRN1b Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Neurexin 1 beta/NXRN1b protein
Human Neurexin 1 beta
(Ala51-Ser363)
Accession # NP_620072		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala51

Predicted Molecular Mass

59.9 kDa (monomer)

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated recombinant rat NLGN-1v2 immobilized on a streptavidin-coated plate at 1 µg/mL
(100 μL/well) can bind Recombinant Human Neurexin 1 beta/NXRN1b Fc Chimera with an apparent Kd <4 nM.

Formulation, Preparation and Storage

5268-NX
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurexin 1 beta/NXRN1b

The alpha and beta forms of Neurexins 1-3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 1 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 1 beta contains only the sixth LNS domain and no EGF-like domains (1 - 3). Mature human Neurexin 1 beta is a 70 kDa glycosylated protein with a 313 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (2, 4, 5). Within comparable regions of the ECD, human Neurexin 1 beta shares 99% aa sequence identity with mouse and rat Neurexin 1 beta. It shares 42% and 68% aa sequence identity with the ECDs of human Neurexin 2 beta and 3 beta, respectively. Neurexin 1 beta isoforms are differentiated by a 30 aa insertion at alternative splice site 4 (SS4), numbered according to splice sites in the longer alpha Neurexins (4, 5). This recombinant protein does not include the SS4 insertion. Neurexin 1 beta is expressed presynaptically at neuronal contacts and interacts with postsynaptic Neuroligins 1-4. The presence of the SS4 insertion significantly weakens this interaction, particularly with Neuroligins 1 and 4 (6-12). Binding affinity is also dependent on the splicing pattern of the Neuroligin (7, 10, 11). Within the presynaptic terminal, Neurexin 1 beta is required for the recruitment of scaffolding proteins and synaptic vesicles (13). Its interaction with Neuroligins 1, 3, and 4 induces the development of glutamatergic postsynaptic terminals containing NMDA receptors, while its interaction with Neuroligin 2 promotes GABAergic postsynaptic development (10, 12, 14, 15). The presence of the SS4 insertion reduces the ability of Neurexin 1 beta to promote glutamatergic contacts but is required for the development of Neuroligin 2/GABAergic contact development (10, 12).

References

  1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
  2. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
  3. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
  4. Rowen, L. et al. (2002) Genomics 79:587.
  5. Ushkaryov, Y.A. et al. (1992) Science 257:50.
  6. Ichtchenko, K. et al. (1995) Cell 81:435.
  7. Comoletti, D. et al. (2006) Biochemistry 45:12816.
  8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  9. Arac, D. et al. (2007) Neuron 56:992.
  10. Chih, B. et al. (2006) Neuron 51:171.
  11. Boucard, A.A. et al. (2005) Neuron 48:229.
  12. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.
  13. Dean, C. et al. (2003) Nat. Neurosci. 7:708.
  14. Nam, C.I. and L. Chen (2005) Proc. Natl. Acad. Sci. 102:6137.
  15. Graf, E.R. et al. (2004) Cell 119:1013.

Alternate Names

NXR1B, NXRN1b

Entrez Gene IDs

9378 (Human); 18189 (Mouse); 60391 (Rat)

Gene Symbol

NRXN1

UniProt

NP_620072

Additional Neurexin 1 beta/NXRN1b Products

Product Documents for Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Neurexin 1 beta Fc Chimera Protein, CF

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