Recombinant Human Neurocan Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6508-NC

R&D Systems, part of Bio-Techne
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6508-NC-050
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Key Product Details

Source

CHO

Accession #

O14594

Conjugate

Unconjugated

Applications

Bioactivity

View all Neurocan Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Neurocan protein
Glu23-Cys1321, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu23

Predicted Molecular Mass

141.5 kDa

SDS-PAGE

140-290 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.
When 5 x 104 cells per well are added to recombinant human Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 50-200 ng/mL.

Formulation, Preparation and Storage

6508-NC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurocan

Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C-type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and
C-terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6 - 8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10 - 15).

References

  1. Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842.
  2. Prange, C.K. et al. (1998) Gene 221:199.
  3. Rauch, U. et al. (1991) J. Biol. Chem. 266:14785.
  4. Matsui, F. et al. (1994) Neurochem. Int. 25:425.
  5. Rauch, U. et al. (1992) J. Biol. Chem. 267:19536.
  6. Asher, R.A. et al. (2000) J. Neurosci. 20:2427.
  7. Matsui, F. et al. (1998) Brain Res. 790:45.
  8. Abaskharoun, M. et al. (2010) Brain Res. 1327:6.
  9. Shen, L.H. et al. (2008) Glia 56:1747.
  10. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  11. Sango, K. et al. (2003) Exp. Neurol. 182:1.
  12. Akita, K. et al. (2004) Biochem. J. 383:129.
  13. Grumet, M. et al. (1994) J. Biol. Chem. 269:12142.
  14. Milev, P. et al. (1996) J. Biol. Chem. 271:15716.
  15. Rauch, U. et al. (2004) Matrix Biol. 22:629.

Alternate Names

CSPG3, NCAN, NEUR

Entrez Gene IDs

1463 (Human); 13004 (Mouse); 58982 (Rat)

Gene Symbol

NCAN

UniProt

O14594

Additional Neurocan Products

Product Documents for Recombinant Human Neurocan Protein, CF

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Product Specific Notices for Recombinant Human Neurocan Protein, CF

For research use only

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