Recombinant Human Neuroligin 1v2 (-ssB)/NLGN1v2 (-ssB), CF

R&D Systems, part of Bio-Techne | Catalog # 6446-NL

R&D Systems, part of Bio-Techne
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6446-NL-050
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Key Product Details

Source

NS0

Accession #

NP_055747

Structure / Form

Noncovalently-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuroligin 1/NLGN1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Neuroligin 1/NLGN1 protein
Gln46-Ser677, Deletion: aa 279-287, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln46 predicted

Predicted Molecular Mass

70.3 kDa (monomer)

SDS-PAGE

90-100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When recombinant human Neuroligin 1 is immobilized at 5.0 μg/mL, recombinant human Neurexin 1 beta Fc Chimera (Catalog # 5268-NX) binds with an apparent KD < 10 nM.

Formulation, Preparation and Storage

6446-NL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 1/NLGN1

Neuroligin 1 (NLGN1 or NL1) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1). Mature human Neuroligin 1 is an N- and O-glycosylated 120 kDa molecule that consists of a 649 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 125 aa cytoplasmic tail (2). In rat, insertion of 20 aa at splice site A and/or 9 aa at splice site B within the ECD results in NLGN1A, NLGN1B, or NLGN1AB isoforms while the NLGN1(-) isoform has neither insertion (3). This recombinant protein lacks both the splice site A and splice site B inserts and corresponds to the human equivalent of rat NLGN1(-). A protein lacking both insertions has also been predicted in chimpanzee (Accession # XP_001166442). Within the ECD, human NLGN1(-) shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 1, respectively. Neuroligin 1 expression is restricted to postsynaptic nerve terminal membranes where it associates into dimers and tetramers (5 ‑ 7). NLGN1B and NLGN1AB are enriched at excitatory (glutamatergic) contacts, whereas NLGN1A is enriched at inhibitory (GABAergic) contacts and NLGN1(-) is expressed at both (3). The interaction of Neuroligin 1 with presynaptic membrane Neurexins promotes the maturation of presynaptic as well as postsynaptic structures (3, 4, 8 ‑ 11). Neuroligin 1 isoforms are selective in which Neurexin isoforms they bind: NLGN1B and NLGN1AB preferentially interact with Neurexin 1 beta lacking the splice site 4 insert, while NLGN1A and NLGN1(-) bind Neurexin 1 alpha and Neurexin 1 beta irrespective of the SS4 insert (12). Neurexin 1 beta (-SS4) additionally binds to LRRTM2 to trigger a second mechanism that promotes excitatory synapse development (13, 14). Neuroligin 1 mediated synaptogenesis is also enhanced by its direct binding to Thrombospondin 1 (15).

References

  1. Sudhof, T.C. (2008) Nature 455:903.
  2. Ichtchenko, K. et al. (1995) Cell 81:435.
  3. Chih, B. et al. (2006) Neuron 51:171.
  4. Dean, C. et al. (2003) Nat. Neurosci. 7:708.
  5. Comoletti, D. et al. (2006) Biochemistry 45:12816.
  6. Berninghausen, O. et al. (2007) J. Neurochem. 103:1855.
  7. Song, J.-Y. et al. (1999) Proc. Natl. Acad. Sci. 96:1100.
  8. Wittenmayer, N. et al. (2009) Proc. Natl. Acad. Sci. 106:13564.
  9. Jung, S.-Y. et al. (2010) Proc. Natl. Acad. Sci. 107:4710.
  10. Chubykin, A.A. et al. (2007) Neuron 54:919.
  11. Chih, B. et al. (2005) Science 307:1324.
  12. Boucard, A.A. et al. (2005) Neuron 48:229.
  13. Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
  14. Ko, J. et al. (2009) Neuron 64:791.
  15. Xu, J. et al. (2010) Nat. Neurosci. 13:22.

Alternate Names

Nlg, NLGN1

Entrez Gene IDs

22871 (Human); 192167 (Mouse); 116647 (Rat)

Gene Symbol

NLGN1

UniProt

NP_055747

Additional Neuroligin 1/NLGN1 Products

Product Documents for Recombinant Human Neuroligin 1v2 (-ssB)/NLGN1v2 (-ssB), CF

Product Datasheets

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Product Specific Notices for Recombinant Human Neuroligin 1v2 (-ssB)/NLGN1v2 (-ssB), CF

For research use only

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