Recombinant Human Neuroligin 3/NLGN3 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9069-NL

R&D Systems, part of Bio-Techne
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9069-NL-050
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Key Product Details

Source

NS0

Accession #

Q9NZ94

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuroligin 3/NLGN3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Neuroligin 3/NLGN3 protein
Ala39-Ser709 (Ser92Tyr), with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala39

Predicted Molecular Mass

76 kDa

SDS-PAGE

87-101 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Neuroligin 3/NLGN3 is immobilized at 2 μg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 = 150-900 ng/mL.

Scientific Data Images for Recombinant Human Neuroligin 3/NLGN3 Protein, CF

Recombinant Human Neuroligin 3/NLGN3 Protein Bioactivity

Recombinant Human Neuroligin 3/NLGN3 Protein Bioactivity

When Recombinant Human Neuroligin 3/NLGN3 (Catalog # 9069-NL) is immobilized at 2 µg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 of 150-900 ng/mL.

Formulation, Preparation and Storage

9069-NL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 3/NLGN3

Neuroligin 3 (NLGN3) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1, 2). Mature human Neuroligin 3 is an approximately 120 kDa molecule that consists of a 672 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 118 aa cytoplasmic tail (3). Alternative splicing generates additional isoforms with 20 aa or 40 aa deletions in the ECD (3-5). Within the ECD, human Neuroligin 3 shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 3. It is expressed during development on astrocytes and Schwann cells (6) as well as on neurons where it localizes to synaptic membranes and post-synaptic densities of both glutamatergic and GABAergic synapses (7). It associates in cis with Neuroligin 1 and Neuroligin 2 and trans-synaptically with Neurexin-1 beta, -2 beta, and -3 beta that lack the splice site 4 insertion (3, 7). A soluble form of the Neuroligin 3 ECD can be released by neurons and promotes glioma cell proliferation (8). Mutations in Neuroligin 3 enhance neuronal dendritic branching, alter both excitatory and inhibitory synaptic activity (9-12), and are associated with the development of autism spectrum disorder (13, 14).

References

  1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
  2. Mackowiak, M. et al. (2014) Pharmacol. Rep. 66:830.
  3. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  4. Philibert, R.A. et al. (2000) Gene 246:303.
  5. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
  6. Gilbert, M. et al. (2001) Glia 34:151.
  7. Budreck, E.C. and P. Scheiffele (2007) Eur. J. Neurosci. 26:1738.
  8. Venkatesh, H.S. et al. (2015) Cell 161:803.
  9. Etherton, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:13764.
  10. Foldy, C. et al. (2013) Neuron 78:498.
  11. Tabuchi, K. et al. (2007) Science 318:71.
  12. Gutierrez, R.C. et al. (2009) Neuroscience 162:208.
  13. Jamain, S. et al. (2003) Nat. Genet. 34:27.
  14. Rothwell, P.E. et al. (2014) Cell 158:198.

Alternate Names

ASPGX1, AUTSX1, HNL3, NL3, NLGN3

Entrez Gene IDs

54413 (Human)

Gene Symbol

NLGN3

UniProt

Q9NZ94

Additional Neuroligin 3/NLGN3 Products

Product Documents for Recombinant Human Neuroligin 3/NLGN3 Protein, CF

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Product Specific Notices for Recombinant Human Neuroligin 3/NLGN3 Protein, CF

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