Skip to main content

Recombinant Human Nicotinamide N-Methyltransferase/NNMT, CF

R&D Systems, part of Bio-Techne | Catalog # 7736-MT

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
7736-MT-010

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human Nicotinamide N-Methyltransferase/NNMT protein
Met1-Leu264, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

30 kDa

SDS-PAGE

25-29 kDa, reducing conditions

Activity

Measured by its ability to methylate nicotinamide.
The specific activity is >65 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7736-MT
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Nicotinamide N-Methyltransferase/NNMT

NNMT (Nicotinamide N-Methyltransferase) is a cytosolic enzyme that catalyzes the N-methylation of nicotinamide and other pyridines using S-adenosyl-L-methionine (AdoMet) as the methyl group donor (1, 2). The enzyme is highly expressed in liver (1) but is also detected in other organs. NNMT plays a significant role in nicotinamide metabolism (3) and in the detoxification of xenobiotics. The association with thyroid cancer and renal carcinoma makes NNMT useful as a tumor biomarker.

References

  1. Cantoni, G.L. et al. (1951) J. Biol. Chem. 189:203.
  2. Aksoy S. et al. (1994) J. Biol. Chem. 269:14835.
  3. D’Souza J. et al. (1980) Xenobiotica 10:151.

Long Name

Nicotinamide N-Methyltransferase

Alternate Names

Nicotinamide NMethyltransferase, NNMT

Entrez Gene IDs

4837 (Human)

Gene Symbol

NNMT

UniProt

Additional Nicotinamide N-Methyltransferase/NNMT Products

Product Documents for Recombinant Human Nicotinamide N-Methyltransferase/NNMT, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Nicotinamide N-Methyltransferase/NNMT, CF

For research use only

Loading...
Loading...
Loading...