Recombinant Human NKG2E Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9759-NK

R&D Systems, part of Bio-Techne
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9759-NK-050
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Key Product Details

Source

HEK293

Accession #

Q07444-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all NKG2E Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human NKG2E protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human NKG2E
(Glu98-Ser240)
Accession # Q07444-1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

43 kDa

SDS-PAGE

52-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD94 (Catalog # 9270-CD) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human NKG2E binds with an ED50 of 1-7 μg/mL.

Scientific Data Images for Recombinant Human NKG2E Fc Chimera Protein, CF

Recombinant Human NKG2E Fc Chimera Protein Binding Activity

Recombinant Human NKG2E Fc Chimera Protein Binding Activity

When Recombinant Human CD94 (Catalog # 9270-CD) is coated at 1 µg/mL, Recombinant Human NKG2E Fc Chimera (Catalog # 9759-NK) binds with an ED50 of 1-7 µg/mL.

Formulation, Preparation and Storage

9759-NK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2E

Human NKG2E (NK cell Group 2 isoform E; Killer cell lectin-like receptor subfamily C, member 3) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). Human NKG2E consist of a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 147 aa extracellular domain (ECD). Within the ECD, human NKG2E shares 40% sequence identity with mouse NKG2E. NKG2E-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (7, 8). Triggering the NKG2E-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (1, 7, 9). Over-expression of KLRC3/NKG2E in glioblastomas may play a major role in glioblastoma aggressiveness and recurrence (10).

References

  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi, I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  8. Kaiser, B.K. et al. (2005) J Immunol 174:2878.
  9. Bellón, T. et al. (1999) J Immunol 162:3996.
  10. Cheray, M. et al. (2017) J Cell Mol Med 21:244.

Long Name

Natural Killer G2E

Alternate Names

killer cell lectin-like receptor subfamily C, member 3, NK cell receptor E, NKG2-E type II integral membrane protein, NKG2-E-activating NK receptor, NKG2ENKG2-E

Entrez Gene IDs

3823 (Human); 58179 (Mouse)

Gene Symbol

KLRC3

UniProt

Q07444-1

Additional NKG2E Products

Product Documents for Recombinant Human NKG2E Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human NKG2E Fc Chimera Protein, CF

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