Recombinant Human NPRB/NPR2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9725-NR

R&D Systems, part of Bio-Techne
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9725-NR-050
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Key Product Details

Source

NS0

Accession #

P20594-1

Conjugate

Unconjugated

View all NPRB/NPR2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human NPRB/NPR2 protein
Arg23-Ile458, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg23

Predicted Molecular Mass

49 kDa

SDS-PAGE

60-85 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9725-NR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRB/NPR2

Human Natriuretic Peptide Receptor-2 (NPR2), also known as NPRB, ANP-RB or guanylyl Cyclase-B, is a member of the guanylyl cyclase family of proteins. NPR2 is a type I transmembrane glycoprotein that contains a 436 amino acid extracellular domain (ECD) (aa 23‑458) for ligand binding, and a 569 amino acid cytoplasmic domain that contains both a protein kinase domain and a carboxyl-terminal guanylate cyclase domain. NPR2 is expressed most highly in in bone, brain, fibroblasts, heart, kidney, liver, lung, uterine, and vascular smooth muscle tissue (1). NPR2 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B type natriuretic peptide), and NPR2 is the principal receptor of CNP (C type natriuretic peptide) (1, 2). Ligand binding to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (2). NPR2 pathway play a critical role in regulation of skeletal growth (3), and patients with single defect NPR2 alleles are statistically shorter than the average population (4). Over the extracellular domain, human NPR2 is 97% and 96% identical to mouse and rat NPR2, respectively.

References

  1. Potter, L.R. et al. (2009) Handb Exp Pharmacol 191:341.
  2. Chang, M.S. et al. (1989) Nature 341:68.
  3. Tsuji, T. and Kunieda T. (2005) J Biol Chem 280:14288.
  4. Olney, R.C. et al. (2006) J Clin Endocrinol Metab 91:1229.

Long Name

Natriuretic Peptide Receptor B

Alternate Names

AMDM, ANPb, ANPRB, ECDM, GC-B, GUC2B, NPR2, NPRBi, SNSK

Entrez Gene IDs

4882 (Human); 230103 (Mouse); 116564 (Rat)

Gene Symbol

NPR2

UniProt

P20594-1

Additional NPRB/NPR2 Products

Product Documents for Recombinant Human NPRB/NPR2 Protein, CF

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Product Specific Notices for Recombinant Human NPRB/NPR2 Protein, CF

For research use only

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