Recombinant Human NrCAM Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2034-NR

R&D Systems, part of Bio-Techne
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2034-NR-050
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Key Product Details

Source

NS0

Accession #

Q14CA1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all NrCAM Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human NrCAM protein
Human NrCAM
Leu30-Asn600 (Ala526Pro)
Accession # Q14CA1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu30

Predicted Molecular Mass

90 kDa (monomer)

SDS-PAGE

120-130 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Y-79 human retinoblastoma cells.
The ED50 for this effect is 0.8-4 μg/mL.

Formulation, Preparation and Storage

2034-NR
Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NrCAM

NrCAM, also known as Bravo, belongs to the L1 family of cell adhesion molecules which also includes L1CAM, Neurofascin, and CHL-1/L1CAM-2 (1). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4-5 fibronectin type III-like domains in their extracellular domain. L1 family cell adhesion molecules are expressed primarily in the nervous system where they share overlapping functions in controlling axonal growth and guidance (2). Mature human NrCAM is an approximately 200 kDa molecule that consists of a 1143 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains followed by 5 fibronectin type III domains; a 23 aa transmembrane segment, and a 114 aa cytoplasmic domain (3). Within the region of Ig-like domains, human NrCAM shares 92% aa sequence identity with mouse and rat NrCAM. Alternative splicing generates additional isoforms with deletions in the juxtamembrane region of the ECD plus short deletions near the N-terminus, between the 2nd and 3rd Ig-like domains, or following the 6th Ig-like domain. A 140 kDa soluble fragment of the ECD can be released by proteolytic cleavage (4, 5). NrCAM is expressed on cerebellar granule neurons, retinal ganglion cells (RGC), star pyramidal cells in visual cortex, thalamocortical axons, and glial cells (4, 6-11). It is found on both axons and dendritic spines (7, 9). NrCAM mediates homophilic adhesion as well as heterophilic adhesion with Contactin, Contactin-2/TAG1, Neurofascin, PTP beta zeta, and Integrin  alpha4 beta1 (5, 12-15) and also interacts with Neuropilin-2, Plexin A3, and EphB2 (8-10). Depending on its interacting partners, NrCAM can promote or inhibit axon and neurite extension (6, 7, 11, 15) and mediate Semaphorin 3F induced neuronal growth cone collapse (9, 10). NrCAM plays an important role in the development of normal vision by regulating RGC axon pathfinding and mapping to the visual cortex (7, 8, 10). It is up-regulated in papillary thyroid carcinomas and the shed form can promote tumorigenesis (5, 16).

References

  1. Sakurai, T. (2012) Mol. Cell. Neurosci. 49:351.
  2. Stoeckli, E.T. and L.T. Landmesser (1995) Neruon 14:1165.
  3. Lane, R.P. et al. (1996) Genomics 35:456.
  4. Sakurai, T. et al. (2001) J. Cell Bio. 154:1259.
  5. Conacci-Sorrell, M. et al. (2005) Cancer Res. 65:11605.
  6. Faivre-Sarrailh, C. et al. (1999) J. Cell Sci. 112:3015.
  7. Zelina, P. et al. (2005) Development 132:3609.
  8. Dai, J. et al. (2013) PLoS One 8:e73000.
  9. Demyanenko, G.P. et al. (2014) J. Neurosci. 34:11274.
  10. Demyanenko, G.P. et al. (2011) J. Neurosci. 31:1545.
  11. Feinberg, K. et al. (2010) Neuron 65:490.
  12. Mauro, V.P. et al. (1992) J. Cell Biol. 119:191.
  13. Sakurai, T. et al. (1997) J. Cell Biol. 136:907.
  14. Lustig, M. et al. (1999) Dev. Biol. 209:340.
  15. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  16. Gorka, B. et al. (2007) Br. J. Cancer 97:531.

Long Name

Neuronal Cell Adhesion Molecule

Alternate Names

Bravo

Entrez Gene IDs

4897 (Human); 319504 (Mouse)

Gene Symbol

NRCAM

UniProt

Q14CA1

Additional NrCAM Products

Product Documents for Recombinant Human NrCAM Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human NrCAM Fc Chimera Protein, CF

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