Recombinant Human NRG1 Isoform SMDF Protein
R&D Systems, part of Bio-Techne | Catalog # 378-SM
Discontinued Product
378-SM has been discontinued.
View all Neuregulin-1 Isoform SMDF products.
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Neuregulin-1 Isoform SMDF protein
Leu117-Glu296, with a C-terminal 6-His tag
Leu117-Glu296, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu117
Predicted Molecular Mass
20.2 kDa
SDS-PAGE
25-45 kDa, reducing conditions
Activity
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 1-5 ng/mL.
The ED50 for this effect is 1-5 ng/mL.
Formulation, Preparation and Storage
378-SM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Neuregulin-1 Isoform SMDF
The heregulin (also known as neuregulin) family of cytokines is comprised of multiple secreted or membrane-bound isoforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. All heregulin family members share an epidermal growth factor (EGF)-like domain ( alpha- or beta-variant) that interacts with the erbB family of tyrosine kinase receptors. NRG1 Isoform SMDF is a heregulin isoform containing a C-terminal EGF-like domain ( beta-variant) and a unique N-terminal sequence that lacks an Ig-like domain which is present in all other known heregulins. NRG1 Isoform SMDF also lacks a transmembrane domain and the cytoplasmic tail. NRG1 Isoform SMDF expression has been found to be restricted to the nervous system. It is likely that NRG1 Isoform SMDF may play an important role in neural-specific functions.
References
- Yarden, Y. and D. Wen (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
- Meyer, D. et al. (1997) Development 124:3575.
Alternate Names
Neuregulin1 Isoform SMDF, SMDF
Gene Symbol
NRG1
UniProt
Additional Neuregulin-1 Isoform SMDF Products
Product Documents for Recombinant Human NRG1 Isoform SMDF Protein
Product Specific Notices for Recombinant Human NRG1 Isoform SMDF Protein
For research use only
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