Recombinant Human OCIL/CLEC2d Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10506-OC

R&D Systems, part of Bio-Techne
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10506-OC-050
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Key Product Details

Source

HEK293

Accession #

Q9UHP7.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all OCIL/CLEC2d Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human OCIL/CLEC2d protein
MD Human IgG1
(Pro100-Lys330)		 IEGR Human OCIL/CLEC2d
(Ala58-Val191)
Accession # Q9UHP7.1
N-terminus C-terminus

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

42 kDa

SDS-PAGE

50-60 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD161 Fc Chimera Protein (Catalog # 7448-CD) is immobilized at 10 μg/mL (100 μL/well), the concentration of Recombinant Human OCIL/CLEC2d Fc Chimera that produces 50% of the optimal binding response is approximately 1.5-9 μg/mL.

Scientific Data Images for Recombinant Human OCIL/CLEC2d Fc Chimera Protein, CF

Recombinant Human OCIL/CLEC2d Fc Chimera Protein Binding Activity.

When Recombinant Human CD161 Fc Chimera Protein (7448-CD) is immobilized at 10 μg/mL (100 μL/well), the concentration of Recombinant Human OCIL/CLEC2d Fc Chimera (Catalog # 10506-OC) that produces 50% of the optimal binding response is approximately 1.5-9 μg/mL.

Formulation, Preparation and Storage

10506-OC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCIL/CLEC2d

C-type lectin domain family 2 member D (CLEC2D), also known as lectin-like NK cell receptor,as lectin-like transcript 1 (LLT-1) and osteoclast inhibitory lectin (OCIL), is a member of the C-type lectin like (CTL) superfamily of natural killer cell receptors (1, 2). Human OCIL/CLEC2D is highly glycosylated and consists of a cytoplasmic domain, a type II transmembrane domain, a stalk region and a C-type lectin-like (CTL) in the extracellular domain (ECD). Within the ECD, mature human OCIL/CLEC2D shares 45% and 43% amino acid sequence identity with mouse and rat OCIL/CLEC2D, respectively. At least six different isoforms of OCIL/CLEC2D, resulting from alternatively spicing, have been reported (1, 2). OCIL/ CLEC2D plays a role in immunotherapy, inflammation and tissue injury and is expressed mainly on activated lymphocytes (NK cells, T cells, B cells) and antigen presenting cells (1-6).OCIL/CLEC2D forms a disulfide linked homodimer that acts as the only ligand for NKRP1A (CD161) and inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion (1,6,7). OCIL/CLEC2D preferentially binds high molecular weight sulfated glycosaminoglycans and blocks osteoclast differentiation (1, 4).

References

  1. Marrufo, A. et al. (2018) Am. J. Cancer Res. 8:1050.
  2. Germain, C. et al. (2010) J. Biol. Chem. 285:36207.
  3. Mathew, S. et al. (2016) Oncotarget 7:68650.
  4. Lai, J. et al. (2020) Immunity 52:123.
  5. Varaden, D. et al. (2019) Eur. J. Obstet. Gynecol. Reprod. Biol. X. 3:100039.
  6. Sun, Y. et al. (2019) J. Cancer Metastasis Treat. 5:80.
  7. Skalova, T. et al. (2015) Acta. Crystallogr. D. Biol. Crystallogr. 71:578.

Long Name

Osteoclast Inhibitory Lectin

Alternate Names

CLEC2D, LLT1

Entrez Gene IDs

29121 (Human); 93694 (Mouse)

Gene Symbol

CLEC2D

UniProt

Q9UHP7.1

Additional OCIL/CLEC2d Products

Product Documents for Recombinant Human OCIL/CLEC2d Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human OCIL/CLEC2d Fc Chimera Protein, CF

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