Recombinant Human ODC1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10316-OD

R&D Systems, part of Bio-Techne
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10316-OD-020
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Key Product Details

Source

E. coli

Accession #

P11926

Conjugate

Unconjugated

Applications

Enzyme Activity

View all ODC1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human ODC1 protein
Asn2-Val461
with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

52 kDa

SDS-PAGE

49 kDa, under reducing conditions

Activity

Measured by its ability to convert ornithine to putrescine.
The specific activity is >750 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human ODC1 His-tag Protein, CF

Recombinant Human ODC1 His-tag Protein Enzyme Activity

Recombinant Human ODC1 His-tag Protein Enzyme Activity

Recombinant Human ODC1 His-tag Protein (Catalog # 10316-OD) is measured by its ability to convert ornithine to putrescine.
Recombinant Human ODC1 His-tag Protein SDS-PAGE

Recombinant Human ODC1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human ODC1 His-tag (Catalog # 10316-OD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 49 kDa under reducing conditions.

Formulation, Preparation and Storage

10316-OD
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ODC1

Ornithine Decarboxylase/ODC1 is a pyridoxal phosphate (PLP)-dependent amino acid decarboxylase enzyme that catalyzes the conversion of ornithine into putrescine in a committed and rate-limiting step for polyamine synthesis.  Each 51 kDa enzyme subunit contains a PLP-binding N-terminal domain and a C-terminal domain. Two active sites are formed through dimerization interfaces of the N- and C-terminal domains of opposing subunits (1). Although the enzymatically active form is homodimeric, the dimer is in rapid equilibrium due to weak association of the monomers and allows regulation of ODC1 activity through the binding of antizymes (Az) and antizyme inhibitors (AzIN) as regulatory proteins (2). ODC1 plays a crucial role in regulating polyamine levels (3) associated with cell growth, proliferation, immunity (4), and differentiation. High levels of polyamines and ODC1 are associated with cancer (5). ODC1 is a gene target of the ras and myc oncogenes (6, 7) while also capable of regulating myc expression through putrescine production (8) implicating its role as an oncogene in cancer. Mutation of ODC1 has also been shown to cause developmental delays in Bachman-Bupp syndrome (9). Consequently, ODC1 is a pharmacological target of interest (5) in a growing number of applications such as in Bachman-Bupp Syndrome (9), iron deficiency (10) and multiple cancers including osteosarcoma (11), neuroblastoma (7), breast (12), lung adenocarcinoma (13), hepatocellular carcinoma (14), and endometrial cancer (15).

References

  1. Bae, D. H. et al. (2018) Biochem. Biophys. Acta. Gen. Subj.1862:2053.
  2. Wu, H. Y. et al. (2015) PNAS 112:11229.
  3. Liu, Y. C. et al. (2011) PLoS One 11:e26835.
  4. Latour, Y. L. et al. (2019) Amino Acids [epub ahead of print] (PMID 31016375).
  5. Sivashanmugam, M. et al. (2017) Biomed. Pharmacother. 86:185.
  6. Pegg, A. E. (2006) J. Biol. Chem. 281:14529.
  7. Bachmann, A. S. and D. Geerts. (2018) J. Biol. Chem. 293:18757.
  8. Tabib, A. et al. (1994) Biochem. Biophys. Res. Commun. 202:720.
  9. Bupp, C. P. et al. (2018) Am. J. Med. Genet. 176:2548.
  10. Saletta, F. et al. (2010) Mol. Pharmacol. 77:443.
  11. Weicht, R.R. et al. (2018) Med. Sci. (Basel) 6: E65.
  12. Fattahi, S. et al. (2018) Cell Mol. Biol. 64:97.
  13. Lam, S.K. et al. (2018) Oncol. Rep. 40:1994.
  14. Ye, Z. et al. (2019) Onco. Targets Ther. 12:4081.
  15. Kim, H.I. et al. (2017) PLoS One. 12:e0189044.

Long Name

Ornithine Decarboxylase 1

Alternate Names

ODC

Entrez Gene IDs

4953 (Human); 18263 (Mouse)

Gene Symbol

ODC1

UniProt

P11926

Additional ODC1 Products

Product Documents for Recombinant Human ODC1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human ODC1 His-tag Protein, CF

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