Recombinant Human P-Cadherin Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 861-PC
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human P-Cadherin protein
| Human P-Cadherin (Asp108 - Gly654) Accession # CAA45177 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Asp108
Predicted Molecular Mass
87.4 kDa
SDS-PAGE
120-130 kDa, under reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells.
When 1 x 105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.
When 1 x 105 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.
Optimal concentration depends on cell type as well as the application or research objectives.
Reviewed Applications
Read 1 review rated 4 using 861-PC in the following applications:
Formulation, Preparation and Storage
861-PC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: P-Cadherin
Placental (P) - Cadherin (PCAD) is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human P-Cadherin is an 829 amino acid (aa) protein with a 26 aa signal sequence and an 803 aa propeptide. The mature protein begins at aa 108 and has a 548 aa extracellular region, a 23 aa transmembrane region, and a 151 aa cytoplasmic region. The human and mouse mature PCAD proteins share 87% homology.
References
- Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
- Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Alternate Names
CAD3, Cadherin-3, CDH3, CDHP, HJMD, PCAD, PCadherin
Gene Symbol
CDH3
UniProt
Additional P-Cadherin Products
Product Documents for Recombinant Human P-Cadherin Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human P-Cadherin Fc Chimera Protein, CF
For research use only
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