Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1224-PL

R&D Systems, part of Bio-Techne
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1224-PL-01M
1224-PL-100
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Key Product Details

Source

NS0

Accession #

Q9BQ51.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all PD-L2/B7-DC Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PD-L2/B7-DC protein
Human PD-L2
(Leu20-Pro219)
Accession # Q9BQ51.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

49 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant human (rh) PD-L2 at 1 µg/mL (100 µL/well) can bind  rhPD-1 with a linear range of 7.5-500 ng/mL.

Reviewed Applications

Read 3 reviews rated 5 using 1224-PL in the following applications:

Scientific Data Images for Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF

Recombinant Human PD‑L2/B7‑DC Fc Chimera Protein SEC-MALS.

Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL) has a molecular weight (MW) of 120.3 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Surface plasmon resonance sensorgram of human PD-L2 protein binding to human PD-1

Binding of Human PD-1 to PD-L2/B7-DC by surface plasmon resonance (SPR).

Recombinant Human PD-L2/B7-DC Fc protein (Catalog # 1224-PL) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human PD-1 His protein (8986-PD) was measured at a concentration range between 6.0 nM and 3.05 uM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=0.274 uM.

Formulation, Preparation and Storage

1224-PL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. Alternative splicing generates additional isoforms that lack the second Ig-like domain and may be substituted and truncated following the first Ig-like domain (4). PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3, 5, 6). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is up-regulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (7-11). PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 12, 13). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (14). In asthma, PD-L2 suppresses IL-5 and IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (9, 11).

References

  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
  3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
  4. He, X.-H. et al. (2004) Acta Biochim. Biophys. Sinica 36:284.
  5. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
  6. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
  7. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
  8. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
  9. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
  10. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
  11. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
  12. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
  13. Shin, T. et al. (2003) J. Exp. Med. 198:31.
  14. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.

Long Name

Programmed Death-1 Ligand-2

Alternate Names

B7-DC, Butyrophilin-like Protein, CD273, PDCD1LG2, PDL2

Entrez Gene IDs

80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)

Gene Symbol

PDCD1LG2

UniProt

Q9BQ51.2

Additional PD-L2/B7-DC Products

Product Documents for Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human PD-L2/B7-DC Fc Chimera Protein, CF

For research use only

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